Preparation Of Inorganic Carbon Nanomaterials And Their Application In Analytical Sensors

Because of its unique surface effect,quantum tunneling effect,small size effect and the quantum size effect,the nanomaterials show excellent performance in photoelectric property and are widely used in the analysises.Because of the unique sp,sp2,sp3 hybridized form of carbon atoms,the carbon nanomaterials(CNMs)exhibit a wide variety of forms.Recently,the CNMs have been widely applied in information,energy,biotechnology and environmental protection.The structure,dimensions,shape,size and other factors of CNMs have a great influence on their properties.Thus,the regulation of the synthesis of CNMs is an important part of the carbon nano-technology development,but also the basic to explore the properties and applied research of CNMs.In addition,most of the CNMs have a wealth of carboxyl and hydroxyl groups,or those groups could be easily modified,which provide the CNMs with good biocompatibility.The good biocompatibility of carbon nanomaterials and their composites are commonly used in biosensors,which lead to the wide synthesis and utilization of those materials.In this paper,we investigated the bioanalysis applications of carbon shell,carbon nanotubes,graphene and their nanocomposites.In order to low the detection limit and broaden the detection range of the sensors,our main research is as follows:1.We designed and synthetized two kinds of materials including carbon coated Fe3O4(Fe3O4-C),the nanocomposite of carbon nanotube carbon and coated Fe3O4(MWCNTs-Fe3O4-C).The photoelectric property of the Fe3O4-C and MWCNTs-Fe3O4-C were investigated by Multi-parameter Chemiluminescence Analyzer and Electronchemical Workstation.The results showed that the two kinds of nanomaterials were with good electroluminescence property.In addition,the obtained MWCNTs-Fe3O4-C was applied in an immunosensor for the detection carcinoembryonic antigen.2.Based on the study of Fe3O4-C,we prepared carbon nanocrystals(CNCs)and assembled CNCs modified Fe3O4-C(Fe3O4-C-CNCs)through a layer-by-layer self-assembly process of CNCs The obtained Fe3O4-C-CNCs were found with good photoluminescence and biocompatibility properties,and were applied as platform of carcinoembryonic antigen primary antibody.Similarly,carcinoembryonic antigen secondary antibody and glucose oxidase were covalently bonded to silica nanoparticles.In addition,a duel-flow photoluminescence flow cell was produced.In the prepared immunosensor,after the injection of glucose,the hydrogen peroxide produced and as a result the photoluminescence was enhanced,and the carcinoembryonic antigen could be detected.3.We prepared the ZnO quantum dots(QDs)through a sol-gel method.And,the ZnO QDs was assembled on the surface of Fe3O4-C and prepared the ZnO QDs coated Fe3O4-C(Fe3O4-C-ZnO).The named magnetic nanocomposites were found with good electrochemiluminescence property.In addition,gold nanoparticles functionalized MWCNTs were prepared to be the supporter of glucose oxidase.With the help of an electrochemiluminescence cell,the prepared nanomaterials were applied in an immunosensor.4.We assembled the ZnO QDs on the surface of graphene oxide,and obtained the ZnO QDs coated graphene oxide(GO-ZnO).In addition,a novel kind of "dioscorea batatas bean"-like silver nanoparticles(AgNPs)was prepared.Due to the rough property of the AgNPs surfaces,the nanoparticles showed good localized plasmon resonance.At last,a DNA sensor based on the localized plasmon resonance-enhanced photoluminescence was prepared.