| Traditional Chinese medicine of Yuyetang is a famous prescription for treatment of Xiaoke disease,Yuyetang and other prescriptions from Yuyetang have good auxiliary hypoglycemic effects.The changed prescription of Yuyetang was guided by compatibility theory of traditional chinese medicine for tonifying Qi,nourishing Yin,and promoting blood circulation for removing blood stasis and developed with reference of Yuyetang,which consisted of 8 kinds of herbs:Radix Astragali,Rhizoma Dioscoreae,Radix Codonopsis,Radix Ophiopogonis,Radix Puerariae lobata,Rhizoma Anemarrhenae,Radix Angelicae Sinensis,Flos rosae rugosae.DR1564 strain of Caenorhabditis elegans carrying a mutation in the daf-2 gene to construct diabetes model in this experiment,which can grow normaly in 15?.However,DR1564 strain will develop insulin resistance,glucose tolerance,and diabetes disease in 22?.Yuyetang and its separated complex prescritions,changed prescriptions of Yuyetang and its separated complex prescritions,Shenqi hypoglycemic granules and Xiaoke pill to treat the diabetic nematodes in22? respectively,then account for the percentage of the adult in total nematodes number.Yuyetang and changed prescrition of Yuyetang has the best effect in reducing the dauer among the other group of its separated complex presicritions.The high dose of changed prescrition of Yuyetang had better effect in reducing the dauer,but the Yuyetang,Shenqi hypoglycemic granules and Xiaoke pill had different degree of inhibition on growth and development of Caenorhabditis elegans.According to the material characteristics and functional factors analysis of changed prescrition of Yuyetang,the water extraction,drying technology,the process of water extraction,drying technology,the preparation process was screened and optimized.Combined with the actual operation environment,process parameters was formulated,under which guidance the 50000 capsuls were maked.The content of crude polysaccharide was3.2g/100 g,which was measured by phenol sulfuric acid method.The mouse acute toxicity test indicated that changed Yuyetang-auxiliary hypoglycemichealth foods maximum tolerance was higher than 40.4g/kg,and significantly higher than the standard of health food specifications for the determination of the toxicology results(greater than or equal to 10g/kg).The fasting blood glucose of normal mice always were 7.5 mmol/L while has finished the oral intra of 30 days,which showed the security of the changed Yuyetangauxiliary hypoglycemic health food.The changed Yuyetang-auxiliary hypoglycemic health food was gived the alloxan induced mice model for 30 days.The high dose group could significantly decreased the level of fasting blood glucose in alloxan induced diabetic mice.Given glucose for 0 h,0.5 h and 2 h,blood glucose of high-dose group was lower than that of model group(P<0.05).The changed Yuyetangauxiliary hypoglycemic health food can improve the alloxan induced symptoms of polydipsia,polyphagia,polyuria,decrease weight loss,and improve the index of thymus and spleen in mice with significant dose dependently.High-fat diet combined with dexamethasone induced rats model,of which the blood glucose level,lipid level and insulin level were reduced by changed Yuyetang-auxiliary hypoglycemic health food for a period of 35 days(P<0.05).Changed Yuyetang-auxiliary hypoglycemic health food can reduce index of liver and kidney and mitigate the degree of dramatic changes in the body weight of rats with dose-dependent,as well as reduce the rat peritoneal relative fat weight.The effect on improvement of blood glucose and insulin level of changed Yuyetang-auxiliary hypoglycemic health food combined with metformin was better than metformin alone(P < 0.05).This study showed that Caenorhabditis elegans model provide high-throughput screening platform for prescriptions of auxiliary hypoglycemic health food.Some theoretical and experimental reference for auxiliary hypoglycemic health food of changed Yuyetang were provided. |
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