Study On Preparation And Adsorption Properties Of Sucrose And Nystose Trihydrate Imprinted Polymers

Molecularly imprinted polymers have three-dimensional cavities with multiple points which match with spatial structure of template molecule and have "memory" function to the imprinting molecule.FOS has many good physiological functions,such as regulating intestinal flora,improving lipid metabolism,lowering cholesterol and blood lipids,protecting liver,promoting absorption of minerals and so on.It is widely used in food and health products.FOS contains glucose,sucrose and a small amount of fructose using sucrose as material.The digestible sugar is not good for diabetes,fat and angiocarpy.The existence of them has effect on physiological fiinctions of FOS.Standard substances of l-kestose,nystose trihydrate and IF-Fructofranosylnystose should be needed when FOS in food is analyzed.Standard substances are very expensive because of relying on foreign imports.Therefore,we prepared imprintrd polymers of sugar and then studied adsorption properties of them.It is significant for separation and purification of FOS.The main contents and results of the paper are explained as follows:1.Sucrose imprinted polymers was prepared by using acrylamide as a functional monomer in this paper.The effect of single-factor experiments on adsorption capacity of MIPs was investigated.On the basis of single-factor experiments,an orthogonal experiment was carried.Structure and morphology of MIPs were characterized by FT-IR and SEM.The results showed that the optimum polymerization reaction conditions were the molar ratio of sucrose and functional monomer in 1:8,the reaction time in 18 h,the reaction temperature at 60?,40 mg of AIBN,15 mmol of EGDMA,the mixture of 9 mL DMSO and 1 mL chloroform as solvent.The FT-IR and SEM results showed that there was interaction between template molecule and functional monomer.2.The adsorption property of sucrose imprinted polymers was investigated in the paper.The results showed that the adsorption equilibrium time was about 240 min;there were two different binding sites in sucrose imprinted polymers,the Kd and Q_max of high affinity binding sites were 1.34 × 10^-2 mg/mL and 23.36 mg/g,the KD and Q_max of lower affinity binding sites were 0.214 mg/mL and 126.84 mg/g,imprinting factor of sucrose imprinted polymers was about 1.7,sucrose imprinted polymers can effectively distinguish between sucrose and other sugar and have high adsorption capacity,high selectivity and specific recognition for template molecule.3.Nystose trihydrate imprinted polymers was prepared by using acrylamide as a functional monomer in this paper.The effect of single-factor experiments on adsorption capacity of MIPs was investigated.On the basis of single-factor experiments,an orthogonal experiment was carried.Structure and morphology of MIPs were characterized by FT-IR and SEM.The results showed that the optimum polymerization reaction conditions were the molar ratio of nistose trihydrate and functional monomer in l:8,the reaction time in 24 h,the reaction temperature at 65 ?,40 mg of AIBN,25 mmol of EGDMA,the mixture of 9 mL DMSO and 1 lL chloroform as solvent.The FT-IR and SEM results showed that there was interaction between template molecule and functional monomer.4.The adsorption property of nystose trihydrate imprinted polymers was investigated in the paper.The results showed that the adsorption equilibrium time was about 240 min;there were two different binding sites in nistose trihydrate imprinted polymers,the KD and Q_max of high afinity binding sites were 1.22 × 10^-2 mg/mL and 17.23 mg/g,the KD and Q_max of lower affinity binding sites were 0.162 mg/mL and 71.21 mg/g,imprinting factor of nystose trihydrate imprinted polymers was about 1.7,nystose trihydrate imprinted polymers can effectively distinguish between nystose trihydrate and other sugar and have high adsorption capacity,high selectivity and specific recognition for template molecule.