Preparation Of Nano-metal Materials From Biogenic Materials For Glucose Sensing Research

Biogenic materials play a crucial role in the development and research of nano-metal materials.They play important roles in the delivery and release of medicines,the synthesis of bacteriostatic materials,bio-imaging and catalyst preparations.Biogenic materials can provide abundant,natural and characteristic chemical functional groups?-COOH,-OH,-NH₂,-SH etc.?for the synthesis of nanomaterials.This can effectively avoid some problems in the synthesis of nano-metal materials?templates,toxic reductant?.Moreover,the synthesized nano-metal materials have good bio-compatibility,high bio-degradability and bio-safety characteristics,which have greatly increased people's interest in their research.Traditional glucose sensors are made from enzymes.However,the properties of the enzyme and the conditions of the test restrict its development.To this end,the introduction of nano-metal materials can well solve this problem of the enzyme glucose sensor,and provide better technical support for the demands of non-enzymatic glucose sensor.With the assistance of biogenic materials to construct nano-materials is a simple,green,and effective strategy for the preparation of nanomaterials without additional template and activator.With the advantages of green,easy obtaining and renewability,it has attracted many scientific researchers'attention.Traditional glucose sensors are made with enzymes.However,the properties of the enzyme and the conditions of the test restrict its development.To this end,the introduction of nano-metal materials can well solve the problem of the enzyme glucose sensor,and provide better technical support for the demand of non-enzymatic glucose sensors.Selecting biogenic materials to constructnano-materials is a simple,green,and effective method for preparing nanomaterials without additional template and activator.With the advantages of green,easy to get,and renewable,it has attracted many scientific researchers'attention?In this paper,the Lilium brownie var.polysaccharide,chitosan extracted from biological resources,and the grinding liquid of Lily were used to prepare nanocomposites with different structures and morphologies in a green and simple way,and then applied to the research of glucose sensing.The main contents are as follows:?1?Pd₃Ag composite nanomaterials were prepared by microwave-assisted treatment of the mixture of Lilium brownie var.polysaccharide,palladium acetate and silver nitrate.By the physical characterization methods of Transmission electron microscopy,X-ray diffraction and X-ray photoelectron spectroscopy,it was proved that Pd and Ag were successfully reduced to form Pd₃Ag alloy composite nanomaterials under certain conditions.Then,the glassy carbon electrode was modified with Pd₃Ag alloy composite nanomaterial,and applied in the glucose sensing by electrochemical method.The results show that Pd₃Ag alloy nanocomposites have good stability and sensitivity with a wide linear range(sensitivity:77.20?A mM^-11 cm^-2,1²0 mM,the detection limit:114.4?M,S/N=3).?2?The elemental Cu was reduced from copper acetate by chitosan in microwave-assisted condition,and the obtained elemental Cu was then used to replace palladium acetate making the Cu core-shell nanocomposite materials coated by Pd.The existence and distribution of elements were demonstrated by Scanning electron microscope-energydispersivespectroscopy,X-raydiffractionandX-ray photoelectron spectroscopy measurements.High angle annular dark field scan and linear scan proved that Pd and Cu formed core-shell structure.The glucose sensing was investigated by electrochemical measurements,and the results show that the nanocomposite has good anti-interference property.In addition,it possesses two good linear range(0.1¹ mM,sensitivity:1.53?A mM^-11 cm^-2,the detection limit:27?M,S/N=3;1¹0 mM,sensitivity:23.00?A mM^-11 cm^-2,the detection limit:52.7?M,S/N=3).?3?The third part directly uses the biological raw material-lily grinding liquid on the basis of the former two experiments?using fine polysaccharides?.The carbon support material was prepared by hydrothermal method in a reaction kettle.Then Ag was supported on the prepared-material to obtain carbon-supported Ag electrode composite.The Fourier transform infrared spectroscopy,X-ray diffraction and X-ray photoelectronspectroscopymeasurementsdemonstratedtheformationof carbon-based materials and the successful supporting of Ag on them.The modified electrode was used in the glucose sensing test,and the experimental results show that the linear range of glucose is 0.1⁰.9 mM,R²=0.996,with almost no response to interference.