Study Of Adhesion Characteristics Of Lycium Barbarum Polysaccharides And Probiotics

In this study,the crude extract of Lycium barbarum polysaccharides(LBP)was obtained from Lycium barbarum L.by water extraction and the crude polysaccharides were purified.The adhesion performance of probiotics was evaluated based on the Caco-2 cell in vitro evaluation model.The evaluations were performed by changing culture conditions such as concentrations of LBP and cells,pH,and culture time,and using antibiotics to simulate changes in the gastrointestinal environment to explore changes in probiotic adhesion properties.Finally,by extracting,separating and purifying cell-bound Exopolysaccharides(c-EPS),the components of LBP and the cell wall polysaccharides of the strain were detected,and the relationship between LBP and cell adhesion characteristics was explored.The following results were obtained:1?The adhesion properties of the seven strains of lactic acid bacteria displayed certain differences,and the adhesion rate was strain specific.2?The LBP solution at a certain concentration range can increased the adhesion rate of lactic acid bacteria,and the LBP concentrations of the seven lactic acid bacteria at the highest adhesion rate were not completely the same.The adhesion rate of Lactobacillus plantarum LP-39 and L.delbrueckii LB-1 increased with increase in bacterial suspension concentration;additionally,pH increased,and the adhesion rate of the strain at 0 to 2 h increased with time.Antibiotics can damage the gastrointestinal tract,which would reduce the adhesion rate of lactic acid bacteria.When LBP was added,the adhesion ability of seven lactic acid bacteria was restored,indicating that LBP can reduce the damage caused by antibiotics to the gastrointestinal flora to some extent.3?The c-EPS of i.acidophilus NCFM and L delbrueckii LB-1 contained galactose,mannose and glucose,similar to the composition of LBP.The findings indicated that LBP could mainly promote cell adhesion due to the similarity of the polysaccharide components.In conclusion,the data confirmed that LBP can improve the adhesion of lactic acid bacteria.In addition,LBP can adjust the intestinal flora imbalance and relieve intestinal damage caused by antibiotics.The findings provide a solid theoretical basis and technical support for the subsequent application of LBP to promote the adhesion and colonization of probiotics in the intestine.