Research And Development Of Compound Mulberry Tea And Its Hypoglycemic Effect

Diabetes is a worldwide endocrine disorder that has a high incidence,with the improvement of living standards,food refinement,nutrition with a serious imbalance and other eating habits so that the incidence of diabetes increased year by year.Therefore,prevention and treatment of diabetes should pay attention to diet conditioning.For patients with diabetes,with hypoglycemic or hypoglycemic efficacy of food has been a lot of attention.China is a mulberry planting country.Mulberry leaves are rich in a variety of bioactive ingredients,so it is a big available resource.Yam both have tonic effect and medicinal value.In recent years,people's attention to it is getting higher than higher.In order to improve the use value of mulberry leaf yam,the further development of natural food with auxiliary hypoglycemic effect,the research showed that,dry mulberry leaves as the main raw material,Henan yam drying tablets as accessories,has developed a hypoglycemic effect of the compound teabag.After reviewing the literature,preliminary to determine the impact of compound bag tea recipe four main factors,they are the origin of mulberry leaves,mulberry grain size,mulberry leaves and yam mantle complex quality ratio and net weight per pack.By sensory evaluation and alpha-glucosidase in vitro inhibition experiments collaborative filtering out single factor optimum level.The result is mulberry leaves produced in Guangdong,over 20mesh sieve,to a mass ratio of 5:1 and with 20 mesh sieve of the yam particles for complex,tea weight per pack is 4g.Choose to have both significant differences and has three factors of continuity.Respectively,the net weight per pack,mulberry leaf size and mulberry leaf and yam quality ratio.And then using Design-Expect V8.0.6 Box-Behnken experimental design of these three factors.Because the single factor of the optimal level of sensory score are higher than 75 points,so they have a good sensory quality.Response to the 17 groups of experiments through in vitro hypoglycemic inhibition test as the evaluation criteria,and the higher the inhibitory rate of?-glucosidase,the better the level of factor it is.In order to fully consider the interaction between the various factors,through the multiple linear regression and quadratic polynomial regression fitting of each factor,get a clear regression equation:Y=71.45+4.16A-3.50B+0.33C-2.33AB-0.62AC-1.16BC-17.32A~2-7.58B~2-4.77C~2.The results are predicted by variance analysis,the best result is:in the case of mulberry leaf origin in Guangdong,the net weight per bag was 4.14 g,the grain size of mulberry leaves was 17.44 mesh,the mass ratio of mulberry was 5.06:1,and the theoretical inhibition rate was 72.1934%.According to the limitations of the actual operation,the processing conditions of the teabag were modified to be 4.14 g,and the mulberry leaf size was 18 mesh,and the mass ratio of mulberry was 5:1.And then the verification process is carried out according to the optimal extraction process.The results show that the comprehensive effect value is consistent with the theoretical prediction value,which shows that this model can be used to optimize the recipe process of tea leaf tea.Using the best recipe process,crushed dry mulberry leaves,after sieving and 20 mesh yam granules for bagging after bagging,sterilization after packaging to get the finished product.Boiling water brewing tea,collecting tea solution after the rotary evaporator,they are concentrated and concentrated with distilled water into high,medium and low concentration of three sample solution.Mice were injected intraperitoneally with streptozotocin and nicotinamide,and the mice of type 2 diabetes mellitus were established.Successful model mice were divided into two groups.Observe and collect data such as body weight,fasting blood glucose,average diet,average drinking water,then analysis found that compared with the blank control group mice,negative control mice had the slowest weight gain,fasting blood glucose levels have also been at high blood sugar levels,he average die and water were more than the level of blank control group.The best performance in the treatment group was the control group,followed by the high dose group.At the end of the experiment,the middle and the low dose groups showed effect on the weight,fasting blood glucose and average diet and water quality of mice,there is a trend to the level of the indicators of the blank control group.Mulberry leaf and yam teabag did improve the content of HDL-C(high-density lipoprotein)and reduce the content of TG(triglyceride)in type?diabetes mellitus mice,CHO(total cholesterol)and LDL-C(high density lipoprotein)content,reduce the formation of INS(insulin)have a certain role.Through the results of HE staining of liver and kidney tissue in each group,mulberry leaf yam tea bag did not damage the liver tissue of mice,but it had a protective effect on kidney tissue.Obviously,the glomerular volume can be reduced and the number of cells in the glomeruli is reduced.