Preparation Of Chiral Teicoplanin Stationary Phase In HPLC

In this paper,nine chiral stationary phases of teicoplanin for high performance liquid chromatography were prepared to recognize phenylglycine and p-hydroxyphenylglycine.The main work is as follows:(1)Teicoplanin was bonded by 4,4'-diphenylmethane diisocyanate to amino propyl silica gel,and then its derivative was reacted further with p-chlorophenyl isocyanate and m-methylphenyl isocyanate,respectively.Three stationary phases have enantioselective resolution ability for phenylglycine and p-hydroxyphenylglycine in HPLC.(2)Teicoplanin was also bonded using 2,4-toluene diisocyanate to amino propyl silica gel,and its derivative was also reacted respectively with p-chlorophenyl isocyanate and m-methylphenyl isocyanate.Three stationary phases of HPLC can separate the recamates of phenylglycine and p-hydroxyphenylglycine.(3)With 1,6-hexadiisocyanate and p-chlorophenyl isocyanate,two teicoplanin chiral stationary phases were fabricated.Both chiral stationary phases were able to resolve phenylglycine and p-hydroxyphenylglycine.(4)Teicoplanin stationary phase was prepared by 3-isocyanate-propyl triethoxysilane as bonding arm.The optical resolutions of phenylglycine and p-hydroxyphenylglycine were obtained on the stationary phase.