Study On Ganoderma Lucidum Mycelium As Drug Delivery Carrier

The sustained drug release is a process when the pharmaceutically active molecule and the carrier combined by physical or chemical means,and the drug active molecule is continuously and slowly released in the body by diffusion until the drug effect is fully exerted.The drug carriers are important parts of drug delivery systems and play as a major factor affecting drug efficacy.The drug sustained-release carrier plays an important role in improving blood drug concentration levels,reducing the number of administrations,reducing drug toxicity,and improving drug treatment effects.Ganoderma lucidum has both good biocompatibility and stability,and the same effect as traditional Chinese herbal medicine.Therefore,we selected Ganoderma lucidum mycelium with a natural hollow structure as a drug sustained-release carrier.In this paper,we first obtained the micro-scale fibrous Ganoderma lucidum mycelium by grinding and pulverizing the fruit body of Ganoderma lucidum.We then treated the Ganoderma lucidum mycelium with hydrochloric acid.The effects of adding different concentrations of hydrochloric acid on the morphology and structure of Ganoderma lucidum mycelium were also studied in the experiment.Test samples were analyzed through FT-IR,XRD and SEM.We found that Ganoderma lucidum treated by acid showed spongy structure,and no cytotoxicity after MTT assay verification processing.Second,through liquid transplantation,we used Rhodamine B to verify the feasibility model drug experiments,used FT-IR,XRD,SEM and other methods to characterize Ganoderma lucidum,and compared Ganoderma lucidum's ability to load drug and sustained performance after different concentrations of hydrochloric acid.The experimental results showed that the Ganoderma lucidum mycelium treated by 22.2% concentration of hydrochloric acid has good drug-loading performance,which can achieve 157.68 mg / g.Results for drug release experiments under 37? in a simulated body fluid(SBF)buffer system showed that Rhodamine B /Ganoderma lucidum mycelium's release rate can reach 51.4% at 12 h and 56.23% in at 24 h.We then used captopril as a model drug to test drug loading and drug release and used SEM,XRD,FT-IR,etc.method to characterize captopril / Ganoderma lucidum mycelium.The results showed that captopril /Ganoderma lucidum mycelium SBF can reach a release rate of 60.99% in the first 6 h before became gentle and a release rate of 80.34% at 48 h.Finally,we used ibuprofen as a model drug,loaded it into the mycelium of Ganoderma lucidum,and characterized it to monitor its release process in the simulated body fluid.The results showed that ibuprofen / Ganoderma lucidum mycelium in SBF has a release rate of 42.55% in the fist 2 h,59.6% at 7 h and 83.79% at 48 h.E.coli bacteriostasis test showed that the antibacterial effect of ibuprofen/Ganoderma lucidum mycelium sustained-release solution increased with the increase of release time.The test results also showed ibuprofen/Ganoderma lucidum mycelium has a longer-lasting antipyretic effect on febrile rats.