Study On Ganoderma Lucidum Spores As Drug Delivery Carrier

Ganoderma lucidum spores(GLS)are seeds of Ganoderma lucidum and have a naturally formed cavity structure.Due to their non-toxic side effects and good biocompatibility,Ganoderma lucidum spores have potential application value in drug release.In this thesis,GLS was used as a carrier to study its application in drug release,and its feasibility and sustained release function as a drug delivery carrier were explored.The GLS was treated through the physico and chemical method to break the micro-domain and to form caved and external connected Ganoderma lucidum spore microsphere(GLS')and Rhodamine B(RhB)was used to prove the feasibility of the experiment.Ibuprofen(IBU)and tamoxifen citrate(TAM citrate)are selected as model drugs to be assembled with GLS'.They were characterized by UV,X-ray diffraction,infrared spectroscopy,scanning electron microscopy,and we monitored the release process of the assembled drugs.We mainly found these results as follows:(1)After assembled RhB and GLS' and monitored RhB/GLS' in simulated body fluid(SBF),we found the release curve in SBF showed that the cumulative release amount was28.71% at 4 hours,55.27% at 24 hours and 60.96% at 48 hours.We then incubated the GLS and GLS' before and after treatment with NRK-52 E cells.After 72 hours,the cell survival rate did not change significantly,which proved GLS and GLS' both have good biocompatibility that are not affected by treatment process,and can be further used for carrier research of drug sustained release;(2)After assembled Ibuprofen(IBU)with GLS',and the assembly IBU/GLS' was released in vitro in SBF,we found the cumulative release was 31.37% in 6 hours,57.95%in 24 hours and 62.56% in 24 hours.The antibacterial performance of E.coli was analyzed by IBU/GLS' and the results showed that the inhibition zone diameter of IBU/GLS' release solution in 48 hours was much larger than the inhibition zone diameter of the 12 hours release solution.The Sprague Dawley rat fever model was established by dry yeast,to examine the antipyretic effect of the assembly IBU/GLS' on the hyperthermia rat model.The body temperature of the rats in the IBU drug group continued to increase after 7 hours,and the IBU/GLS' group remained to have a significant antipyretic effect at 12 hours.(3)After assembled the TAM citrate with GLS',and monitored the release process of the assembly TAM citrate/GLS' in SBF,we found the release curve in SBF showed that the cumulative release amount was 30.57% at 6 hours,53.29% at 24 hours and 59.37% at 48 hours.The effect of assembled drugs on the proliferation of MCF-7 human breast cancer cells in vitro was examined at the cellular level,and the sustained release properties of GLS' as a drug carrier were further verified.