| Traditional Chinese medicine is the cultural treasure of the Chinese nation.Traditional Chinese medicine has been escorting the Chinese nation's diet for thousands of years.It has unique effects in health care and other aspects.Most of the extracts of traditional Chinese medicines are mixtures,the chemical composition is complex,large volume of the extraction liquid,there are many impurities,the active ingredient content is small and difficult to purify.Therefore,it is of great significance to establish a rapid separation and purification method for effective chemical components in Chinese herbal medicines.The macroporous adsorption resin separation and purification technology can selectively adsorb the effective chemical components in the Chinese medicine and remove the impurity components.he method is widely used in the separation and purification of effective chemical constituents of traditional Chinese medicines with many advantages such as good selectivity,short adsorption time,easy desorption,large adsorption volume,simple regeneration,and no pollution to the environment.In this paper,macroporous adsorption resins were screened according to the adsorption and desorption capacities of macroporous adsorption resins for the active constituents of the Empetrum nigrum L.var.japonicum K.Koch.,Aster tataricus L.f,Rhododendron dauricum L.,Glehnia Littoralis Fr.Schmidt ex Miq.,Poria cocos(Schw.)Wolf..The resin was screened according to the static adsorption and desorption experiments,and the best active ingredients of the above traditional Chinese medicines were purified by using the best type of macroporous adsorption resin.The factors affecting their adsorption,desorption and elution were investigated separately,and the optimal purification conditions were obtained to improve the purity of the active ingredients in traditional Chinese medicine.The main contents are as follows:1.Purification of total flavonoids from Empetrum nigrum L.var.japonicum K.Koch.by macroporous adsorption resinOptimize the high performance liquid chromatography(HPLC)analysis conditions oftotal flavonoids from Empetrum nigrum L.var.japonicum K.Koch.,HPLC method for the determination of total flavonoids from Empetrum nigrum L.var.japonicum K.Koch.was established.Based on HPLC separation method,the extraction process of total flavonoids from Empetrum nigrum L.var.japonicum K.Koch.was optimized.Eight types of macroporous adsorption resins were screened by static adsorption and desorption experiments.Finally,AB-8 macroporous adsorption resin with the best adsorption and desorption properties of total flavonoids from Empetrum nigrum L.var.japonicum K.Koch.was selected for enrichment and purification.The purification process of total flavonoids of Empetrum nigrum L.var.japonicum K.Koch.was further explored,and the optimal static adsorption desorption conditions such as adsorption equilibrium time,desorption solvent,desorption equilibrium time and adsorption isotherm were determined.Then we explored the dynamic adsorption elution conditions such as the sample concentration,maximum sample volume,elution volume,and elution flow rate.Under the optimal experimental conditions,the mass fraction of total flavonoids of Empetrum nigrum L.var.japonicum K.Koch.was increased from 49.16% before purification to 89.59% after purification,which achieved the purpose of purifying the total flavonoids of Empetrum nigrum L.var.japonicum K.Koch..2.Study on Purification of Chemical Constituents from Aster tataricus L.f by Macroporous ResinThe HPLC analysis conditions of the chemical constituents in Aster tataricus L.f were optimized,and the HPLC separation method of the chemical constituents in Aster tataricus L.f was established.The extraction process of Aster tataricus L.f was optimized based on the HPLC separation method.Then,five types of macroporous adsorption resins were screened by static adsorption and desorption experiments.Finally,the AB-8macroporous adsorption resin with the best adsorption and desorption properties of the chemical components in Aster tataricus L.f was selected to enrichment and purification.Further explored the purification process of the chemical constituents of Aster tataricus L.f.The static adsorption time,desorption solvent and static desorption time were optimized,and then the dynamic adsorption elution conditions such as maximum samplevolume,elution volume and elution flow rate were determined.Under the optimal conditions,the mass fraction of the chemical constituents of Aster tataricus L.f was increased from 44.2% to 87% after purification,and the purpose of purifying the chemical components in Aster was achieved.3.Purification of Total Flavonoids of Rhododendron dauricum L.by Macroporous ResinOptimize of HPLC analysis conditions for total flavonoids of Rhododendron dauricum L.,HPLC method for the determination of total flavonoids from Rhododendron dauricum L.was established.Based on HPLC separation method,the extraction process of total flavonoids from Rhododendron dauricum L.was optimized.Five types of macroporous adsorption resins were screened by static adsorption and desorption experiments.Finally,HPD-400 macroporous adsorption resin with the best adsorption and desorption properties of total flavonoids of Rhododendron dauricum L.was selected to enrichment and purification.The purification process of total flavonoids of Rhododendron dauricum L.was further explored,and the static adsorption time,desorption solvent and static desorption time were optimized,and then the dynamic adsorption elution conditions such as maximum sample volume,elution volume and elution flow rate were determined.Under the optimal conditions,the mass fraction of total flavonoids of Rhododendron dauricum L.increased from 33% to 79% after purification,which achieved the purpose of purifying the total flavonoids of Manchurian red.4.Purification of Coumarin from Glehnia Littoralis Fr.Schmidt ex Miq.by Macroporous ResinThe HPLC analysis conditions of Coumarin from Glehnia Littoralis Fr.Schmidt ex Miq.were optimized,and the HPLC separation method of Coumarin from Glehnia Littoralis Fr.Schmidt ex Miq.was established.Then five types of macroporous adsorption resins were screened by static adsorption and desorption experiments.Finally,HPD-826 macroporous adsorption resin with the best adsorption and desorption properties was selected to enrichment and Purification of coumarin from Glehnia Littoralis Fr.Schmidt ex Miq..The purification process of Coumarin from Glehnia Littoralis Fr.Schmidt ex Miq.was further explored.The optimal static adsorption time,desorption solvent and static desorption time were determined.Then the dynamic adsorption elution conditions such as maximum sample volume,elution volume and elution flow rate were optimized.Under the optimal conditions,the mass fraction of coumarin from Glehnia Littoralis Fr.Schmidt ex Miq.increased from 16.4% to 85.7% after purification,indicating that HPD-826 macroporous adsorption resin can effectively purify coumarin from Glehnia Littoralis Fr.Schmidt ex Miq.5.Purification of triterpenoid from Poria cocos(Schw.)Wolf.by macroporous adsorption resinThe HPLC analysis conditions of triterpenoid from Poria cocos(Schw.)Wolf.were optimized,and the HPLC separation method of triterpenoid from Poria cocos(Schw.)Wolf.was established.The extraction of triterpenoid from Poria cocos(Schw.)Wolf.was optimized based on the HPLC separation method.Then,five types of macroporous adsorption resins were screened by static adsorption and desorption experiments.Finally,the HPD-100 macroporous adsorption resin with the best adsorption and desorption properties was selected to enrichment and Purification the triterpenoid from Poria cocos(Schw.)Wolf..The purification process of triterpenoid from Poria cocos(Schw.)Wolf.was further explored,and the optimal static adsorption time,desorption solvent and static desorption time were determined.Then the dynamic adsorption elution conditions such as maximum sanple volume and elution volume were optimized.Under the optimal process conditions,the mass fraction of the triterpenoid from Poria cocos(Schw.)Wolf.was increased from 36% to 67% after purification,and the purpose of purifying the triterpenoid from Poria cocos(Schw.)Wolf.was achieved. |
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