Preparation And Application Of Multifunctional Nanoparticles Based On Prussian Blue

The effective treatment of tumor cancer has always been a major historical problem that plagues human beings.The photothermal therapy?PTT?as a novel cancer treatment has developed recently,which is less invasive and has better therapeutic effects than traditional treatments.In addition,PTT can be applied to the diagnosis and therapy of tumor cells in combination with other types of treatments.Among them,PTT combined with chemotherapy is a typical treatment.In general,the combination of PTT and chemotherapy can achieve synergistic effects.The heat generated by photothermal conversion can promote the release of the drug from the drug carrier and the chemotherapy can compensate the shorts of the discontinuity and poor therapeutic effect of of PTT to large tumors.Therefore,the combination of chemotherapy and PTT can do better than the pure PTT or chemotherapy in tumor ablation.In this paper,we synthesized two types of multifunctional nanocomposites with different structures,Prussian blue?PB?NPs were used as photothermal reagents,and the calcium phosphate?CaP?and silica with pH response performance were coated on the surface of the NPs,respectively.Furthermore,the drug delivery capacity,photothermal conversion ability and tumor cell ablation ability of the NPs were studied.The details are as follows:The PB@CaP core-shell NPs prepared by the microemulsion method are uniform in morphology and size.Subsequently,the PB in the NPs was etched by NaOH solution to finally obtain the mesoporous CaP coated PB NPs?M-PB@CaP?core-shell NPs.The drug loading capacity,drug release behavior,photothermal effect and cytotoxicity of NPs were further studied.The results show that the prepared NPs are excellent photothermal reagents with a photothermal conversion efficiency of up to 26%and good drug carriers with a drug loading efficiency of 100%.In vitro experiments show that the combination of PTT and chemotherapy has a good application prospect.Through a simple synthesis method,we successfully prepared nanocomposites of PB and silica with different special structures..In the experiment,we found that by changing the ratio of water and isopropyl alcohol,the composites of PB and SiO₂ having different morphologies can be obtained.When the ratio of water to isopropanol is 1:10,we can obtain PB/SiO₂ with a Janus structure.In the NPs,the bare PB can provide a strong photothermal conversion capability without being interfered by the SiO₂ at the other side,and the porous silica can be used to deliver the drug.The experimental results show that the prepared Janus PB/SiO₂ NPs have high photothermal conversion efficiency?29%?,ultra-high drug loading efficiency?96%?and pH/near-infrared light dual-responsive drug release.In vitro experiments have further demonstrated,and the efficacy of synergic therapy is much better than either PTT or chemotherapy alone.