The Mechanism Of Cpt1a Tmp And The Efficient Use Of Medicine Ingredients Based On Obesity And Diabetes

With the gradual increase of obesity and diabetes,exploring the related pathological mechanisms and developing food ingredients for specific population have certain research value.In this paper,the mechanism of oligomerization of CPT1A transmembrane protein domains was mainly studied.Secondly,the efficient utilization of medicine ingredients.was preliminarily explored.CPT1A is a rate-limiting enzyme for fatty acid oxidation.Its function depends on the oligomerization of transmembrane domain,but the mechanism of action remains unclear.This experiment simulates the phenomenon of transmembrane oligomerization by CG field and mutates important residues to study the interaction mechanism of oligomers,so as to understand the oligomerization mechanism.Firstly,wild type TM1,wild type TM2 and mutant G7I were constructed to explore the mechanism of dimerization;Wildtype TM2,mutant G7I and mutant G7₁3I were constructed to explore the mechanism of trimerization.Then,the stability of oligomer structure was analyzed by bdist map,cross dihedral angle,residue contact map and RMSD map.The results showed that the TM1 transmembrane domain could not form a dimer.The GXXXG is an important motif for maintaining TM2 TMD dimerization stability.GXXXG and GXXXA are important motifs for maintaining the stable structure of the trimer,while the GXXXG motif is superior to the GXXXA motif to maintain oligomeric structure.Finally,the binding strength of oligomers of wild type and mutant was further analyzed by g_mmpbsa and PFM from the energy point.The results showed that the PMF of WT oligomers was higher than MT G71 and G7₁3I,which indicated that the binding ability between wild-type dimeric and trimeric structural protein chains was stronger than mutant oligomers.The absolute value of binding free energy of dimer is mutant G7I<wild type,the absolute value of binding free energy of trimer is mutant G7₁3I<mutant G7I<wild type,the binding strength of wild type oligomer is strong,and the structure of oligomer is more stable.Two analytical methods further illustrate the conclusion of stability.Medicinal ingredients generally have high sugar content or low effective absorption rate,which is not conducive to obesity and diabetes.Therefore,this paper preliminarily explored how to use biological methods to improve the utilization value of medicinal ingredients,retain beneficial substances,reduce sugar content,and reduce consumption.In this paper,two common ingredients:Gouqi and Ginseng were studied by water extraction and microbial methods.The results showed that pectinase can increase the yield of ginseng water extract,which is more than 15%higher than the original process.Through the analysis of overlapping chromatogram,we found that the pectinase method had no effect on the main functional components of Ginseng;It was found that both pectinase and cellulase were effective and they have better effects than the water extraction method,and the pectinase effect is more obvious.The use of edible lactic acid bacteria and yeast can reduce the "energy sugar" in Gouqi,Gouqi pulp is more suitable for aerobic fermentation of yeast,The content of reducing sugar was reduced by more than 51%in 48 hours,the hypoglycemic effect was good,and the content of protein and total flavonoids remained basically unchanged.