QuEChERS-Chromatography-Mass Spectrometry For Rapid Determination Of Inedible Pigments,Environmental Hormones And Mycotoxins In Capsanthin

With increasingly serious environmental pollution,inedible pigments,environmental hormones,mycotoxins and other harmful substances are widely present in the environment such as soil and air.These harmful substances are easily transferred to foods during the growth,storage,transportation or processing of foods and agricultural products,such as the capsanthin,during it's production process,they are concentrated and enriched along with extraction and refining processes.At the same time,these harmful substances are not easily volatilized and degraded during the extraction process.Therefore,capsanthin has a certain amount of these harmful substances.When capsanthin is added to food as a food additive,it will contaminate foods and cause food quality and safety problems.In food testing,the food matrixes being tested are complex,the detection interferences are large,and there are many types of these harmful substances from the environment and their contents are low.Therefore,it is necessary to research the detection methods of inedible pigments,environmental hormones and mycotoxins in plant extracts and carry out methodological verification.A method for simultaneous determination of 9 kinds of inedible pigments in capsanthin was developed by QuEChERS pretreatment method coupled with ultra-high performance liquid chromatography-tandem mass spectrometry?UPLC-MS/MS?under multiple reaction monitoring?MRM?mode.Capsanthin samples were extracted with acetonitrile-water,then sodium chloride was added for salting out,the extracting liquids were cleansed by C₁₈8 adsorbent,and the supernatants were analyzed by ultra liquid chromatography-tandem mass spectrometry.According to the results,the limits of detection?LOD?and limits of quantitation?LOQ?were 0.2?g/kg¹.5?g/kg and 0.6?g/kg⁵.0?g/kg respectively,the linear ranges were 0.1?g/L¹0?g/L for basic orange2,basic orange 21,basic orange 22 and solvent yellow 34 HCl,0.5?g/L²0?g/L for sudan I,sudan II,sudan III,sudan IV and rhodamine B chloride,the linear correlation coefficients?R²?were more than 0.9950;and under the three levels,the average recoveries of 9 kinds of inedible pigments were between 69.6%and 92.5%,and the relative standard deviations?RSD?were between 2.8%and 8.5%.The method is rapid,sensitive and accurate,and is suitable for rapid detection of inedible pigments in large quantities of natural plant extract samples.Based on the QuEChERS pretreatment method coupled with gas chromatography-mass spectrometry?GC-MS?,a method for the simultaneous determination of 17 kinds of phthalate esters?PAEs?in capsanthin was developed.Capsanthin samples were extracted with acetonitrile,and then sodium chloride and anhydrous magnesium sulfate were added for salting out.After the extracting liquids were cleansed by florisil,the supernatants were analyzed by GC-MS.The limits of detection?LOD?ranged from 0.2 mg/kg⁰.5 mg/kg.DMP,DEP,DAP,DIBP,DBP,BMPP,DPP,DHXP and DCHP were in the range of 0.5mg/L²0 mg/L;DMEP,DEEP,BBP,DBEP,DEHP,DPhP,DNOP and DNP were in the range of 2 mg/L⁴0 mg/L.And the linear correlation coefficients?R²?were more than0.9950.The average recoveries of 17 kinds of PAEs of the three levels were between82.8%and 118.1%,and the relative standard deviations?RSDs?were between 0.12%and7.3%.It is a simple,rapid,accurate and reliable method for the simultaneous detection of multiple PAEs in large quantities of natural plant extract samples.In the experiment,A method for simultaneous determination of 11 kinds of mycotoxins in capsanthin was developed by QuEChERS pretreatment method coupled with UPLC-MS/MS under MRM mode.Capsanthin samples were extracted with acetic acid-acetonitrile-water?1:70:29,V/V?,then sodium chloride was added for salting out,the extracting liquids were cleansed by C₁₈8 adsorbent,the supernatants were analyzed by ultra liquid chromatography-tandem mass spectrometry.The results showed that the linear ranges were 3?g/L¹00?g/L for DON,FB₁,FB₂,T-2,10?g/L²00?g/L for ZEN,and1?g/L⁴0?g/L for AFM₁,AFB₁,AFB₂,AFG₁,AFG₂,OTA,the linear correlation coefficients?R²?were more than 0.9930;the limits of detection?LOD?and limits of quantitation?LOQ?were 1.2?g/kg¹2?g/kg and 3.6?g/kg³6?g/kg,respectively;the average recoveries of 11 kinds of mycotoxins of the three levels were between 80.0%and118.2%,and the relative standard deviations?RSD?were between 1.16%and 9.87%.The method has the advantages of simpleness,speediness,accuracy and wide range of application,it is suitable for rapid detection of multiple mycotoxins in large quantities of commercial samples.