The Study On Extraction,purification,characterization And Activity Of Polysaccharide From Suaeda.glancabunge And Product Development

Suaeda glaucabunge is a plant of the Suaeda genus,which grows in high-salt and hypertonic saline-alkali soils with salt-tolerant growth characteristics in China,and the unique salt-stressed environment has created a variety of active components.Therefore,we study the extraction,purification process,structural identification,antioxidant effect in vitro and development of activated carbon with Suaeda glaucabunge residual based on the analysis of the main nutritional components of the plants.The ultrasonic-assisted extraction method with hot-water is used to extract the polysaccharides based on the research on the factors affecting the extraction.The optimal extraction process of the polysaccharides is ethanol volume fraction of 65%,extraction time of72 min,ultrasonic power of 438 W,and extraction temperature of 85?.Under this optimized process condition,the yield of polysaccharides are about 2.19±0.13%.In the study of decolorization of polysaccharides,the decolorization effects of activated carbon,HZ-800macroporous adsorption resin,hydrogen peroxide and composite bentonite are compared.The decolorization effect is the best when the amount of HZ-800 macroporous resin is 1.8%.And further comparing the deproteinization effect of Savage,salting out,TCA and enzyme-Savage treatments.Obviously,the deproteinization result is best when the alkaline protease addition is7%.At this time,the polysaccharide retention and protein removal rate reach 67.27%and80.71%,respectively.The polysaccharides are subjected to DEAE-52 Cellulose system chromatography to obtain two fractions contained SGP-1 and SGP-2 after decolorization and deproteinization pre-treatments.The absolute molecular weight of crude SGP is 6.6 x 10⁵ g/mol,average particle diameter is 7.953?m and specific surface area is 44234 cm²/mL.Characterization and analysis of the two purified fractions of SGP-1 and SGP-2 are determined.FT-IR analysis proves to be a characteristic polysaccharide structure with?-bonds containing uronic acid groups.According to the SEM test,the structure of SGP-1 upon the appearance shows a tight cluster agglomerate form,while the SGP-2 component is characterized by a broken structure,which the form is irregularly distributed.HPLC analysis shows that SGP-1 includes 9 kinds of monosaccharides contained glucose,mannose,xylose,galacturonic acid,glucuronic acid,fucose,rhamnose,arabinose,galactose,and the corresponding moles,which the ratio is 0.27:0.85:0.21:2.13:0.63:0.92:0.23:1.15:0.43.Besides,SGP-2 contains 6 kinds of monosaccharides of glucose,mannose,galacturonic acid,glucuronic acid,fucose,arabinose,which the corresponding molar ratio is 0.83:0.42:0.56:1.87:0.61:0.63.The ability of·OH clearance,DPPH·clearance,ABTS reducing power,and O^2-clearance of SGP-1,SGP-2 and crude polysaccharides shows a dose-dependent effect in vitro on the antioxidant studies.A bio-based activated carbon is prepared by using H₃PO₄-ZnCl₂ dual activator from the residue Suaeda glaucabunge after extraction.The best process parameters obtained by orthogonal test are H₃PO₄-ZnCl₂ mass fraction of 30%,activation temperature of 500?and activation time of 80 min.Moreover,the average yield of activated carbon prepared under this optimized condition is 43.39%.Obviously,the iodine adsorption value is 865.45mg/g,which is 27.5%higher than that of ordinary commercially available food-grade coconut shell-based activated carbon.