Study On The Chemical Composition And Anti-Acute Lung Injury Activities Of Gnaphalium Hypoleucum,Sauropus Spatulifolius And Scutellaria Baicalensis

Acute lung injury(ALI)is mainly based on diffuse lung injury and pulmonary vascular injury which leads to pathological features of lung inflammatory cell infiltration and pulmonary edema.The pathogenesis of acute lung injury is related to complement hyperactivation and oxidation Stress damage,imbalance of inflammatory factors,etc.This paper aimed to study on anti-inflammatory effects of acute lung injury of three kinds of plants:Gnaphalium hypoleucum D.C.,Sauropus rostratus Beille,and Scutellaria baicalensis Georgi.The research group used natural drug extraction and separation structure identification methods and UHPLC-Q-TOF/MS method to analyze the chemical constituents of three kinds of plants.Part ?.Chemical constituents and activities of Gnaphalium hypoleucum D.C.In this paper,the anti-complement classical pathway activity test was carried out on G.hypoleucum.It was found that the alcohol extract,chloroform layer,ethyl acetate layer and total flavonoids of G.hypoleucum had strong anti-complement activity.Experiments on acute lung injury in mice showed that the extract of G.hypoleucum had a protective effect on acute lung injury in mice,which could significantly reduce the ratio of wet to dry weight of lung,significantly improve pulmonary edema and reduce total protein concentration in alveolar lavage fluid.In this paper,the research group used UHPLC-Q-TOF/MS method to analyze the further chemical constituents of G.hypoleucum and based on the retention time and mass spectrometry signal of the base peak ion chromatogram of the reference,19 compounds were extracted and identified,and most of the resolved compounds were flavonoids and their glycosides.Four of them were consistent with the retention time and mass spectrum of the control,and identified as luteolin-4'-O-?-D-Glucoside,luteolin,apigenin,5,7-dihydroxy-3,8,4'-trimcthoxyflavonc.Part ?.the chemical composition and activity of Sauropus rostratus BeilleOur research group conducted anti-acute lung injury research on S.rostratus.The study found that S.rostratus significantly reduced lung wet-to-dry weight ratio,significantly improved pulmonary edema,and significantly decreased total protein concentration in alveolar lavage fluid,and significantly reduced pro-inflammatory factor TNF-?.IL-6 and IL-1? levels.We used UHPLC-Q-TOF/MS method to analyze the chemical composition of S.rostratus,and the retention time and main mass spectrometry signals of the samples were analyzed.Based on the retention time and mass spectrometry signals of the reference peak ion chromatograms,thirteen compounds were identified from the ethanol macroporous resin elution site(LLY40),mainly phenolic compounds,organic acids,and alkaloids.Part ?.the chemical composition and activity of Scutellaria baicalensis GeorgiIn vitro antioxidant activity test,anti-complement activity test and anti-mouse acute lung injury test were carried out on S.baicalensis;S.baicalensis extract and Baicalin had strong free radical scavenging activity;alcohol extract,petroleum ether layer,chloroform layer,ethyl acetate layer and baicalin have anti-complement activity.Among them,baicalin has stronger anti-complement activity than each extract layer;extract of S.baicalensis has anti-mouse acute lung injury,which significantly improves lung edema in mice and significantly reduces alveolar protein content in the lavage fluid.In this paper,the chemical research of S.baicalensis was carried out,and five flavonoids were isolated and identified from the ethanol extract of S.baicalensis:aspen-6-C-?-L-arabinose-8-C-?-D-glucoside,baicalin,wogonin-7-O-?-D-glucuronide methyl,wogonin and baicalein.