Research On Imaging Of Biological Tissue Based On Raman Spectroscopy

Raman spectroscopy belongs to inelastic scattering that usually occurs in the visible and near-infrared bands.It is a fast,accurate and non-destructive detection technology.Through specific vibration modes,different substances can be identified and then molecular changes can be observed with high specificity by Raman spectroscopy.Because of these advantages,Raman spectroscopy is gradually applied in the field of biochemistry,and a lot of achievements have been made.This thesis is divided into two parts.In the first part,the changes of liver tissue after cadmium contamination is studied by using unlabeled Raman confocal imaging.The normal liver tissue and cadmium poisoning liver tissue samples are collected,frozen and sliced.Then,for all liver tissues,an 100?m?100?m area is selected for Raman mapping.The distribution of important macromolecules in two types of tissues are studied,and the changes in biochemical components of liver tissue are quantitatively described by comparing the regional average Raman spectra.Compare with the control group,the liver strength of cadmium-poisoned mice at 748cm^-1,1128cm^-11 and 1585cm^-11 represent protein significantly decreases,and the intensity of 1082cm^-11 that represents collagen is significantly higher than which of the control group.These show that the degree of fibrosis of liver tissue caused by cadmium poisoning is increased.After confirming the results,we look for a30?m?15?mhepatocyte area for high-resolution Raman imaging.The results shows that lipids and proteins increase significantly in specific parts of liver cells.Confocal Raman imaging of liver tissue provides a unique tool to better understand disease-induced biochemical phenotypic changes in primary liver tissue.Our study provides a valuable reference for in vitro studies on the accumulation and toxicity of cadmium in the human liver.In the second part,we study the changes in the main nutritional components of edible lilies during refrigeration.Lily cultivation is distributed almost everywhere in China.Lily is rich in nutritional value,and it is rich in essential nutrients such as protein,carbohydrates,lipids and mineral elements.Lily polysaccharide contained in lily has extremely high medicinal value to the human body and can improve human immunity.Besides,alkaloids and polyphenols contained in lily can prevent cancer.In this paper,we first measure the Raman spectra of lilies from several different origins.Through the Raman spectra,we can get some information about polysaccharides,proteins and carotenoids in lilies.We can also observe that there are some differences in the content of various substances in different types of lilies.Secondly,we take unearthed fresh Lanzhou lily as the object,and we use Raman spectroscopy to study the changes of various nutritional components of lily scales with the increase of refrigeration time.As time grows,we also find that in the short term,the intensity of some peaks in the lily spectrum will increase,and the starch intensity will decrease.When the storage time becomes longer,the corresponding spectral intensity of each component in the lily will generally decrease,which is consistent with the results reported in related literature.Besides,the study also find that the Raman spectra of the inner layer is significantly different from the outer layer of the same scale.In the outer layer,the intensity of peaks at 1605cm^-1,1640cm^-11 represent amino acid is higher,and the inner layer does not have these peaks.