Study On Preparation Of Taurine From Mussel Meat With Pulsed Electric Field Assisted Enzymatic

Taurine has physiological functions such as protecting myocardium and retina,and has important functions in the fields of anti-oxidation,improving immunity,and promoting lipid absorption.Therefore,it is widely used in medicines and health foods.The Songhua River Basin in Jilin Province of my country is rich in wild river mussel resources,which are rich in taurine,but the utilization rate of the mussel meat is low,resulting in waste of resources.In this paper,we use wild mussel meat as raw material to study the use of ultrasonic technology,ultra-high pressure technology and high-voltage pulsed electric field technology to assist in enzymatic hydrolysis of mussel meat to extract taurine,and separate and purify it to obtain high-purity taurine product,which is natural beef The efficient preparation of sulfonic acid provides theoretical support,and also provides a new way for the deep processing of mussel meat.The enzymatic extraction process of taurine in mussel meat showed that the detection wavelength of taurine was determined by full-scanning mode of ultraviolet spectrophotometry to determine the content of taurine in mussel meat;the results of single factor test and orthogonal test study show that the optimal process parameters for enzymatic extraction are enzymolysis temperature,enzyme addition amount,enzymolysis time and enzymolysis pH are 50?,4000U/g,3h and 7.5,respectively.The extraction rate of taurine in meat was 10.74mg/g.Ultrasound-assisted enzymolysis of taurine extracts showed that the quadratic polynomial regression equation between the taurine extraction rate?Z₁?and enzyme addition?X₁?,ultrasonic time?X₂?,ultrasonic power?X₃?and enzymolysis time?X₄?:Z₁=11.13+0.40X₁-0.049X₂+0.01X₃+0.071X₄-0.080X₁X₂-0.11X₁X₃+0.0075X₁X₄-0.32X₂X₃-0.015X₂X₄+0.11X₃X₄-0.54X₁²-0.43X₂²-0.39X₃²-1.30X₄²;the optimal test conditions for ultrasonic-assisted enzymatic extraction of taurine in mussel meat are:the amount of enzyme added is 4374 U/g,the ultrasonic time is 19 min,the ultrasonic power is200 W,and the enzymatic hydrolysis time is 3 h.The acid extraction rate was 11.21mg/g.The study on the extraction of taurine by ultrahigh pressure assisted enzymolysis of mussel meat showed that the best process parameters of ultrahigh pressure assisted enzymolysis for taurine extraction were:pressure 200MPa,holding time 3min,enzymolysis time 3.5h and enzyme addition amount is 5000U/g,the maximum taurine extraction rate is 11.63mg/g;the order of the effects of various factors on the taurine extraction rate is the enzyme addition amount,pressure holding time,enzymatic hydrolysis time and pressure.High-voltage pulsed electric field assisted enzymolysis of taurine extracts from mussel meat showed that the quadratic polynomial regression equation between the extraction rate of taurine?Z₂?and the electric field strength?Y₁?,pulse number?Y₂?and enzymolysis time?Y₃?:Z₂=13.40+0.12Y₁+0.35Y₂+0.086Y₃+0.83Y₁Y₂-0.052Y₁Y₃-0.082Y₂Y₃-0.31Y₁²-0.51Y₂²-0.27Y₃²;the optimal process parameters of high voltage pulsed electric field assisted enzymolysis for extraction of taurine from mussel meat are electric field intensity 25 kV/cm,10 pulses,and enzymolysis time 2.95h,at which time the maximum taurine extraction rate is 13.77mg/g;the order of the influence of various factors on the extraction rate of taurine is in order of pulse number,electric field strength and enzymatic hydrolysis time.Studies on the separation and purification of taurine from mussel meat showed that after removing impurities,the crude extract of river mussel meat was treated with732 type cation exchange resin,and it was found that when the pH of the column solution was 4.5,the elution rate was 2 mL/min and the injection volume was 8mL,the separation effect of taurine in the crude extract is the best;after concentrating the extract through the resin column,adding 4 times the volume of absolute ethanol,the crystallization and recrystallization are repeated 3 times to obtain taurine with a purity of 98.2%,the product has a taurine yield of 0.61%;the structure of the purified product was identified by infrared spectroscopy,and the results proved to be basically consistent with the structure of the pure taurine reported in the literature.