Preparation And Application Of Electrochemical Biosensor Based On Nanomaterials

Nanomaterials are the cornerstone of the promotion and application of nanotechnology.They exhibit different special properties in different fields and play a mainstay role in the development of science,technology and social progress.Nanomaterials have large specific surface area,excellent mechanical,optical,electrical,magnetic,biocompatibility and catalytic properties,which makes them have broad application prospects in the construction of electrochemical sensors for analysis and detection.The introduction of cerium oxide?CeO₂?nanomaterials into electrochemical sensing has created new opportunities for the development of electrochemical sensors,and has unlimited possibilities for improving the performance of sensors,that is,constructing highly sensitive,highly selective and stable sensors.The charge transfer rate and hole transfer rate of perylene imide?PDI?and its derivatives?PDIs?are higher than that of typical electron hole transfer materials.At the same time,due to its excellent adsorption performance,good chemical structure stability,and thermal stability.As well as its excellent ability to adsorb electrons and good electron mobility,it has also been widely studied and applied in electrochemical catalysis and organic solar cells.Electrochemical biosensors are widely used in the fields of synthesis and modification of nanomaterials,environmental detection and clinical analysis and diagnosis due to their simple structure,convenient fabrication and processing,and electrochemical oxidation-reduction reactions.Based on the advantages of the above nanomaterials in the construction of electrochemical sensors,in this paper,we mainly focus on the synthesis and preparation of new functional nanomaterials,and use their excellent properties to construct electrochemical biosensors to achieve the detection of hydrogen peroxide?H₂O₂?.First,three types of fluorene imide probes with different structures were synthesized,Probe1,Probe2,Probe3,and their structures were characterized;then hydrothermal method was used to synthesize cerium dioxide nanoparticles,and the particle size of the nanoparticles was finally 20 nm;the three types of probes synthesized above were combined with cerium nanoparticles to form new composite nanomaterials,CeO₂@Probe1,CeO₂@Probe2,CeO₂@Probe3,and the composite materials were characterized by morphology and compared with simulated enzyme activities.Among them,CeO₂@Probe1 has the best catalytic performance.Finally,using a three-electrode system,made CeO₂@Probe1 to make electrochemical biology.The sensor uses cyclic voltammetry and current time method to detect different concentrations of hydrogen peroxide.The detection range is 1×10^-5-5×10^-2mol/L?R²=0.998,K_m=11.4 mM?,and the lower detection limit is?LOD?4.2×10^-6mol/L?S/N=3?.The pH of the detection system was optimized,and the anti-interference ability,stability and reproducibility were tested.It has broad application prospects in detecting H₂O₂.