| Triple negative breast cancer is a highly aggressive subtype of breast cancer,due to lack of estrogen receptor,progesterone receptor and human epidermal growth factor receptor expression.Currently,there is no safe and effective therapeutic strategy to treat it.Resveratrol is natural polyphenolic compound extracted from plants,which is abundant in grapes,peanuts and other dietary foods.However,the poor water-solubility and low bioavailability of resveratrol extremely limit its bioactivity and clinical application.In order to improve the water-solubility of resveratrol and therefore enhance its anti-tumor activity against triple-negative breast cancer,in this study,we synthesized oxidized mesoporous carbon nanoparticles to construct a nano-delivery system of resveratrol,and explored its effect on triple-negative breast cancer cells in vitro.1.Mesoporous carbon nanoparticles were synthesized by one-step aqueous solution method and then modified it by oxidation.The particle size,mesoporous parameters and morphology of synthesized mesoporous nanoparticles were determined by dynamic light scattering experiment,gas adsorption analyzer,transmission electron microscope and scanning electron microscope.Furthermore,the hydrophilicity and dispersity of mesoporous carbon nanoparticles were evaluated by dispersion analysis.The results showed that the synthesized oxidized mesoporous carbon nanoparticles have uniform particle size distribution,large specific surface area and pore capacity,good hydrophilicity and dispersion,which can be used as the delivery carrier of resveratrol.2.Using oxidized mesoporous carbon nanoparticles as the carrier,to select the optimal drug loading conditions,and then the drug loading rate of resveratrol was determined by ultraviolet spectrophotometry.The particle size,mesoporous parameters and morphology of drug loaded nanoparticles were determined by dynamic light scattering experiment,gas adsorption analyzer,transmission electron microscope and scanning electron microscope,and the surface groups and thermogravimetric losses of resveratrol loaded mesoporous carbon nanoparticles were studied by infrared spectrometer and thermogravimetric analyzer.By X-ray diffraction and differential scanning calorimetry,the distribution state of resveratrol loaded into nanoparticles was analyzed.Furthermore,the solubility and release properties of resveratrol after loaded into mesoporous carbon nanoparticles were evaluated.The results showed that the synthesized oxidized mesoporous carbon nanoparticles had high drug loading rate,uniform particle size and morphology,what's more,the nanoparticles significantly improved watersolubility and release property of resveratrol.3.We studied the anti-tumor effect of resveratrol loaded mesoporous nanoparticles on triple negative breast cancer in vitro.The cellular uptake capacity of nanoparticles was evaluated by laser confocal microscopy and flow cytometry,and by MTT experiment,we evaluated the biocompatibility of the blank nanoparticles and the inhibitory efficiency of resveratrol on triple negative breast cancer cells before and after loading into the nanocarrier.Furthermore,Annexin V-FITC staining assay,tunel staining assay and western blot assay were used to investigate the pro-apoptotic effect of resveratrol on triple negative breast cancer cells.The results showed that the oxidized mesoporous carbon nanoparticles synthesized in this study had good biological safety,could improve the uptake capacity of cells to drugs,and therefore enhanced the bioactivity on triple negative breast cancer.As a non-toxic and biocompatible drug delivery system,the mesoporous carbon nanocarrier can effectively improve the poor water-solubility and low bioavailability of resveratrol.Compared with free drug,resveratrol loaded into mesoporous carbon nanoparticles exhibited greater effects on inhibiting cell activity and inducing cell apoptosis.This study provides a new idea for the treatment of triple negative breast cancer. |
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