Extraction Process Optimization Of Rg3 Saponins From Red Ginseng And Its Application In Health Care Liquor

Ginseng,commonly known as King of Herbs,is rich in many active ingredients.Ginsenosides is one of the most effective biologically active substances with the effects of lowering blood sugar,anti-fatigue,and anti-cancer,etc.Among them,saponins are more useful but very rare,especially Rg3.When subjected to high-temperature processing,available saponins content will decrease,thereby decreasing both the economic and the use-vale of ginseng.In avoidance of this,it is necessary to adopt a processing technique that will retain or increase the saponin content of ginsenosides,particularly Rg3.In this study,high-temperature hydrothermal method was used for red ginseng preparation in a reaction kettle,and the ginsenoside extraction was done with the aid of ultrasonic.Hence,to facilitate the production of healthy red ginseng wine,the processing was done under an optimum condition,and the content of ginsenoside(Rg3 saponins)was determined using high-performance liquid chromatography.The test results are as follows:According to a single-factor and response surface optimization test results,the optimal processing conditions for high-temperature hydrothermal method were determined the steaming(temperature was 150°C,the steaming time was 1h,and the drying temperature was 65°C)The content of Rg3 saponin increased significantly to 643.03 ± 0.31 μg/ml,which was 1.21 times of that of the traditional steaming method,but the time was 1/3 of that of the traditional method.According to the single-factor and response surface optimization test results,the optimal extraction process parameters of Rg3 saponin in red ginseng were determined as follows: material-liquid ratio 1:34(g / m L),ultrasonic time 35.5 min,ultrasonic power 330 W and with a maximum yield of 4.80%content of Rg3 saponins in red ginseng.It was again determined that the preparation parameters of red ginseng liquor were: alcohol content 46% vol,red ginseng extract content 200 mg,ultrasonic time 30 min.The processed red ginseng was extracted with ethanol and blended with white wine to produce healthy red ginseng liquor.The prepared red ginseng liquor had a golden yellow color,clear and transparent,harmonious aroma,natural and comfortable,and with a soft alcoholic body,with total sugar content of 2.3 g / 100 m L,and total acidic content of 5.3 g / 100 m L,and solid soluble content of 10 g / 100 m L.In summary,the reaction kettle high-temperature hydrothermal processing method was the easy way for achieving a substantial amount of retained Rg3 saponin,and its steaming time was significantly shorter than the traditional steaming method.The processing efficiency was high,which was convenient for industrial production.