Research And Application Of Luminol Chemiluminescence System Catalyst

The light emitted by chemical reaction is called chemiluminescence?CL?.The CL method can be widely used for the determination of a variety of samples due to the simple instrument,easy operation,high analysis efficiency,high sensitivity,and no interference from background scattered light.So far,it has become a broad and practical analytical method in many fields such as molecular biology,clinical and pharmacy,environmental science,and food analysis.Luminol and its derivatives,bipyridine ruthenium complexes,peroxyoxalate derivatives,acridinium ester,and oxidized light-emitting substances(KMnO₄,Ce⁴⁺)are commonly used chemiluminescence reagents.Among them,luminol has been reported to produce CL under alkaline conditions in 1928,and has become the most widely used CL reagent due to its excellent performance.Under the action of various different oxidants,the CL system can be divided into luminol-hydrogen peroxide?luminol-H₂O₂?CL system,luminol-potassium ferricyanide?luminol-K₃Fe?CN?₆?CL system,luminol-periodate and other systems.However,the quantum yield of some CL reactions is low,and the intensity of chemiluminescence produced cannot meet the needs of detection,resulting in the development of CL being limited.In order to further develop the practical application value of CL,finding new catalysts,illuminants or energy acceptors has become a current research hotspot.So far,the use of catalysts has gradually changed from metal ions,horseradish peroxidases to nanomaterials and novel metal composite materials.Therefore,starting from the synthesis of high-efficiency catalysts,the catalysts with good catalytic performance were prepared to enhance the luminol-H₂O₂ CL intensity.By optimizing the test conditions,the detection of thiocyanate ions?SCN^-?in the environmental system and the glucose content in the biological samples were realized.This paper is divided into three chapters:Chapter one:Introduction.Firstly,the development of CL method and the commonly used luminescent reagents are briefly described.Secondly,the application of several different types of catalysts in CL systems are described in detail.Finally,several techniques currently used in combination with CL are described.Chapter two:A water-soluble substance?Hemin-mPEG?was synthesized at room temperature using Hemin and poly?ethylene glycol?methyl ether?mPEG?to catalyze luminol-H₂O₂ CL.This substance not only maintained the excellent catalytic activity of Hemin,but also solved the problem of Hemin insoluble in water.In addition,the mechanism of Hemin-mPEG catalyzed luminol-H₂O₂ reaction to enhance CL strength was investigated.Based on this,a sensitive,fast and simple CL sensor for quantitative detection of glucose and H₂O₂ was established.Chapter 3:Based on the advantages of simple preparation,non-deactivation and good biocompatibility,the core-shell gold platinum nanoparticles?Au@Pt NPs?with catalytic activity of peroxidase were synthesized by seed mediated method to enhance the CL strength of luminol-H₂O₂ reaction.It was found in the study that SCN^- can effectively inhibit the catalytic activity of Au@Pt NPs,leading to a decrease in CL intensity.Based on this phenomenon,a sensitive and efficient CL sensor for quantitative detection of SCN^- was established.