Preparation Of Straw Microbial Fertilizer And Impact On The Growth Of Tobacco

In this study,Nitrogen-fixing bacteria,Organophosphate-hydrolyzing bacteria and Inorganic phosphorus-hydrolyzing bacteria were screened from the plant rhizosphere soil,and they were used with methylotrophic Bacillus which can antagonize Fusarium sp.In solid state fermentation,corn stalks were used as the substrate,a single factor test and response surface method were used to obtain the optimal conditions for the maximum viable bacterial count of solid state fermentation nutrients,and a new type of compound microbial bacterial fertilizer was prepared.At the same time,using the prepared composite microbial bacterial fertilizer tobacco cultivation test,through the determination of tobacco growth characteristics and disease resistance,the application effect was tested.In this paper,the Nitrogen-fixing bacteria N₃,Organophosphate-hydrolyzing bacteria Y₁,and Inorganic phosphorus-hydrolyzing bacteria W₃ were obtained from the plant rhizosphere soil.The 16S rDNA identification results were Arthrobacter sp.ZSH N₃,Enterobacter asburiae ZSH Y₁,and Enterobacter hormaechei ZSH W₃,the deposit numbers of CGMCC are 1.18471,1.18470 and 1.18469 respectively.The plate confrontation culture method was used to screen out the methylotrophic Bacillus KY₂,which has a good inhibitory effect on Fusarium proliferatum,as a biocontrol agent.It is known by the cross method that the Nitrogen-fixing bacteria N₃,the organophosphate-hydrolyzing bacteria Y₁,the Inorganic phosphorus-hydrolyzing bacteria W₃,and the Biocontrol bacteria KY₂ have no antagonism with each other.Corn stalk was selected as the basic solid substrate,and the corn stalk was pretreated by adding biogas slurry and inoculating with Trichoderma reesei RUT-C30 suspension,then inoculated with Nitrogen-fixing bacteria N₃,Organophosphate-hydrolyzing bacteria Y₁,Inorganic phosphorus-hydrolyzing bacteria W₃,Biocontrol bacteria KY₂ mixed bacteria suspension,after fermentation for a certain period of time to prepare the new composite microbial bacterial fertilizer.Through the single factor experiment and response surface method,the optimum conditions for the maximum number of viable bacteria in solid fermentation nutrients were obtained.The fermentation conditions were temperature 30.4?,initial pH 7.7,initial water content 100%,inoculation volume 15.5%,inoculation interval time 12h,fermentation At the time of 120h,the actual effective viable bacteria count of Nitrogen-fixing bacteria N₃,Organophosphate-hydrolyzing bacteria Y₁,Inorganic phosphorus-hydrolyzing bacteria W₃,and Biocontrol bacteria KY₂ reached 2.27×10⁹,3.11×10⁹,7.10×10⁸ and 3.21×10⁹ CFU·g^-1,the total nitrogen and available phosphorus content are 6.07 times and 11.43 times of the raw material.Tobacco was selected as the test plant for pot experiment to verify the efficacy of compound microbial fertilizer.The results of the pot experiment showed that the application of the compound microbial fertilizer can significantly improve the growth characteristics of tobacco plants,promote the photosynthesis of tobacco leaves,and increase the nutritional content of tobacco leaves.It can also effectively increase the nutritional content of potting substrates and improve potting.The microbial community structure of the substrate,and effectively control Fusarium wilt disease of tobacco layer.In summary,the thesis completed the screening and identification of Nitrogen-fixing bacteria N₃,Organophosphate-hydrolyzing bacteria Y₁,Inorganic phosphorus-hydrolyzing bacteria W₃,and Biocontrol bacteria KY₂.Through solid-state fermentation,a new composite microbial bacterial fertilizer was prepared.Through the pot experiment of tobacco,it shows the good effect of compound microbial bacterial fertilizer,which can be further industrialized and popularized.