Preparation And Application Of Several Hemin-based Composites

Hemin has a catalytic activity material with a ability of peroxidase active center.It has belongs to porphyrins,hemoglobin and myglobin prosthetic groups.A series of hemin-based composite materials were synthesized with hemin and other materials having stability with catalytic activity.Improving the excellent properties of hemin will help us to achive further development in life science and medicine sectors also using in the photocatalytic degradation of dyes,glucose electrochemical sensors and hydrogen peroxide electrochemical sensors.In this paper,hemin-based composite materials were synthesized based on hemin as a basic reactant,combined with excellent nanomaterials such as graphene oxide and ferroferric oxide.They were used for photocatalytic degradation of dyes,glucose electrochemical sensors and hydrogen peroxide electrochemical sensors.Hemin-based mateilas are shown to exhibit highly efficient catalytic activity in the innovation study.?1?A hemin-aminated graphene composite?H-NG?was prepared with methylene blue solution?MB?via a one-step hydrothermal method.Influence of visible light irradiation,the efficiency of H-NG to catalyze the degradation of MB solution was achived 96%,which was having significantly higher than the efficiency of aminated graphene and hemin-graphene oxide composite.H-NG materials could better coordinate with the dyes due to the role of amino groups,and enhance the catalyst's adsorption capacity;H-NG could produce·OH oxidatively degrade MB solution because it having the properties of peroxidase;also H-NG reacts with molecular oxygen to formed strong oxidizing nature single-line oxygen ¹O₂ and superoxide anion O₂^-·under visible light condition.These three factors synthesizes the H-NG capable of high efficiency degrade MB solution.?2?A hemin-ferroferricoxide-oxide-reduced graphene oxide?H-FG?composite was prepared via a chemical co-precipitation method,and glucose oxidase?GOD?was immobilized on H-FG to construct an electrochemical glucose sensor based H-FG/GOD.The results indicate that the H-FG with peroxidase could promote the electron transfer between glucose oxidase and the electrode because it has both hemin peroxidase characteristics,and Fe₃O₄ particle stability,biocompatibility and electronic conductivity,and rGO provides good the substrate.Compared with hemin and Fe₃O₄,the electrochemical sensor based on H-FG/GOD was effective.The linear range of the composite modified electrode for glucose detection was 0.3?M-1.3 mM,and its detection limit wass 0.12?M?S/N=3?.The results indicate that the H-FG material improves the selectivity and sensitivity of GOD to glucose,and has a excellent effect on the detection of actual samples.?3?The previously prepared hemin-ferroferricoxide-reduced graphene oxide?H-FG?composite,an electrochemical sensor based on H-FG hydrogen peroxide was constructed.The study showed that H-FG combines the characteristics of Hemin peroxidase,as well as the stability of rGO,biocompatibility and electronic conductivity of Fe₃O₄,making H-FG sensors more sensitive and stable.The experimental results shown that the H-FG with peroxidase activity could directly detect hydrogen peroxide.The linear range of the H-FG hydrogen peroxide electrochemical sensor was 0.25?M-800?M,and the detection limit was 0.10?M?S/N=3?.