Detection Of Typical PPCPs In Drinking Water And Its Effect On Bacterial Community Structure

Drugs and personal care products,one of the emerging micro-pollutants,are widely used in people’s daily lives.Entering the environment through domestic sewage,industrial wastewater,and animal husbandry wastewater to produce pollution.At present,there are different levels of detection in surface water,groundwater and drinking water.In order to explore the effects of PPCPs on the bacterial community structure in drinking water,nine drug PPCPs and four antibiotic PPCPs were selected to study the changes of PPCPs content and bacterial diversity.This paper established a detection method that can simultaneously measure chloramphenicol,erythromycin,oxytetracycline and penicillin G.The pretreatment conditions were optimized to determine the optimum pH of 5,the eluent used 8 mL of methanol,the mobile phase was 0.1% formic acid aqueous solution and acetonitrile,the chloramphenicol was scanned with negative ions,and the other antibiotics were scanned with positive ions.Then,a certain concentration of 9 kinds of drug PPCPs were added to the drinking water for removing residual chlorine and chlorine,and the content and bacterial diversity were measured at different times.It is found through experiments that the attenuation order of nine PPCPs in drinking water with residual chlorine is: ketoprofen>clenbuterol>diltiazem>bezafbrate>nadolol>disopyramide>sulpiride>vera pamil>fluoxetine.In the drinking water supplemented with chlorine,the attenuation order is: verapamil>clenbuterol>diltiazem>nadolol>sulpiride>disopyramide>fluoxetine>ketoprofen>bezafbrate.The main strains in drinking water are Propionivibrio、Sphingomonas 、 Parvularcula and Mycobacterium,and the strains sensitive to pharmaceutical PPCPs are Propionivibrio 、 Alkanindiges 、 Lactobacillus and Paenibacillus.Finally,a pipe network simulation device was set up to add a certain concentration of single antibiotics,and samples were analyzed at different times.Erythromycin and oxytetracycline showed a downward trend with time,chloramphenicol content decreased first and then increased,and penicillin G floated within a certain range.Through the analysis of bacterial community structure of four groups of experiments,it was found that the susceptible strains of chloramphenicol were Bosea、Caulobacter、Altererythrobacter、Novosphingobium;the susceptible strains of erythromycin were Ralstonia、Delftia、Hydrotalea、Massilia、Acinetobacter、Arthrobacter;the susceptible strains of oxytetracycline were Variibacter 、Sphingomonas、Acinetobacter、Caulobacter、Bosea、Aquabacterium;the susceptible strains of penicillin G were Caulobacter 、 Bradyrhizobium 、 Variibacter 、Methylobacterium、Acinetobacter、Bdellovibrio、Aquabacterium、Novosphingobium.