Effects Of Replacement Of Fish Meal By Fermented Soybean Meal On Growth,Immunity And Protein Metabolism In Phoxinus Lagowskii Dybowski

Using fish meal and fermented soybean meal as a protein source,dextrin and flour as a carbohydrate source,corn oil and fish oil as the fat source,cellulose as filler,fermented soybean meal replaced 0.0%(control),16.5%,33.0%,49.5% and 66.0% the fish meal in five isocaloric(total energy of 15.2 MJ / kg)and isonitrogenous(crude protein content of 380.0 g / kg)diets.Each diet was fed to Phoxinus lagowskii Dybowski(initial mean weight,(8.06 ±0.95)g)for 8 weeks.This study was conducted to evaluate the effects of partial replacement of fish meal by soybean meal on growth,non-specific immunity,serum biochemical parameters,protease activity and protein metabolism in Phoxinus lagowskii Dybowski.These results are as follows:1.Effects of replacing fish meal partially in the diet with fermented soybean meal on growth and nutritional composition in the muscle of Phoxinus lagowskii Dybowski.In this study,the effects of replacing fish meal partially in the diet with fermented soybean meal on the growth and feed utilization of Phoxinus lagowskii Dybowski.With fermented soybean meal replacement ratio increased,final mean body weight,weight gain rate,specific growth rate and protein deposition rate of treatment groups showed a downward trend,and compared to that of the control group,these four indicators of 49.5% and 66.0% groups were decreased significantly(P<0.05).Protein efficiency ratio and feed efficiency ratio of 66.0% group were significantly lower than that of the control group(P<0.05).With the increasing replacement ratio of fish meal by fermented soybean meal,the content of crude protein in muscle of 49.5%,66.0% groups was decreased significantly(P<0.05),while there was no significant difference in moisture,crude fat and crude ash(P>0.05).2.Effects of replacement of fish meal by fermented soybean meal in diets on non-specific immunity of Phoxinus lagowskii Dybowski.No significant difference was observed in the SOD and LZM in serum of 16.5% and 33.0%(P>0.05),the SOD and LZM in serum of 49.5% and 66.0% groups were decreased significantly compared to the control group(P<0.05),In this study,fermented soybean meal as a fish meal replacement in diets,while the AKP in serum was not significantly affected in each group(P>0.05).3.Effects of fermented soybean meal as a fish meal replacement in diets on serum biochemical parameters of Phoxinus lagowskii Dybowski.The results showed that the UN of 49.5% and 66.0% groups were increased significantly(P<0.05),and the ALB of 66.0% group was decreased significantly(P<0.05)compared to the control group.The replacement of fish meal fermented soybean meal had no significant effect on SG,TG,CHOL,TP,GLB and A/G in Phoxinus lagowskii Dybowski.4.Effects of fermented soybean meal as a fish meal replacement in diets on protease activity and protein metabolism in Phoxinus lagowskii Dybowski were investigated.When fermented soybean meal replacement ratio in 49.5% and 66.0% groups,proteases activity in middle and posterior intestine were decreased significantly(P<0.05).With the replacement of fermented soybean meal as a fish meal in diets,proteases activity in anterior intestine and hepatopancreas were no significance(P>0.05)and parameters of intestine/body were no significance(P>0.05).In this study,the GOT and GPT in serum of 16.5% and 33.0% groups were no significance compared with the control group(P>0.05),With fermented soybean meal replacement ratio in 49.5% and 66.0% groups,GOT and GPT were increased significantly(P< 0.05).With fermented soybean meal replacement ratio below 49.5%,no significant effect was detected on the growth,non-specific immunity,serum biochemical parameters,protease activity and protein metabolism in Phoxinus lagowskii Dybowski.