| The Peristenus spretus (Chen et van Achterberg, 1997) is a primary parasitoid for mirid bugs. At present, there is little research on the reproductive physiology of it.In this paper, The developmental morphology and ovarian development of the P.spretus pupa were studied. At the same time, we compared the reproductive system,the structure of the ovary and the occurrence of the egg under different temperature feeding conditions. the reproductive system, the structure of ovary and the occurrence and ultrastructure of ovary were studied and observed. The relationship between the morphology, structure and function of the organs in the reproductive system of the wasp was studied. The protein from the ovaries were identified by electrophoresis and proteomics analysis, and the classification of the protein gene product and its biological function were studied.The main results are as follows:1. The P. spretus pupa were observed using optical microscopy: that the wasp is a kind of pheasant parasitic wasps, before the eclosion has begun to develop ovaries and ovarian tube has a small amount of mature eggs. Late stage male and female can be distinguished by either pupal surface color changes or the shape of the end of the abdomen of adults.2. Ovaries of P. spretus were dissected and observed under optical microscopy and electron microscopy in order to explore the reproductive system and egg development model of female wasp. Most of the female ovaries are composed of 6-8 pairs of ovarian tubes. At 23 ? and 15 ?, the number of mature eggs in ovary increased with age, and the total number of eggs in ovary was higher than 15 ? under 23 ?. The basic structure of the ovarian structure was clarified by semi-thin slicing technique. In the initial emergence stage less mature eggs were found, ovarian tube contains a large number of oogonia, the egg is filled with a lot of fat droplets, later with the fat body of the degradation of absorption, the egg gradually newborn some Golgi, mitochondria and other organelles. The vitality of these organelles is also increasing with the increasing of variation. The trophoblast cells and oocytes alternately appear in the eggs, revealing that trophoblast cells provide nutrition to oocytes for development. However, in the development of ovarian eggs throughout the process, we did not find the presence of yolk protein particles and transport process.3. The results showed that there were about 20 protein bands located between 15 and 260 KD, and the protein bands of the ovaries were mainly concentrated in 25 KD-120 KD, identified by the protein spectrum of 1382 proteins were identified, remove the redundant protein identified a total of 1190 non - redundant protein, which in the 80KD tape to identify a section of egg yolk protein peptide fragments. The bioinformatics analysis of ovarian expression protein revealed the functional proteins and biological pathways related to the formation of egg cells in the common circles of P. spretus. Biology path analysis found that cell composition, metabolic process,metabolic process, catalytic activity protein in the ovary is more abundant. Analysis by KEGG pathway revealed that proteins involved in transcriptional transcription were found to be the most abundant in the ovaries by genetic information processing. |
PDF Full Text Request