Application Of Genome And Transcriptome Sequencing Technology To Thalassemia Prevention And Control And Paris Polyphylla Var. Yunnanensis Breeding In Yunnan Area

Genome and transcriptome sequencing technology has been applied to the studies in medicine,agriculture and other areas.To facilitate the prevention and control of high incidence disease thalassemia in Yunnan area,we applied next-generation sequencing(NGS)screening method specifically for the high risk people,the Dai nationality,in Yunnan.Our findings revealed that:(1)A total of 951 samples were screened and the a-&?-thalassemia carrier rate is 8.60%.For ?-thalassemia carriers,the high frequency of CD26 mutations suggested a correlation between CD26 and the environmental adaption of the Dai people.(2)In traditional screens,74.8%of a-thalassemia carriers and 30.5%of composite ?-&?-thalassemia carriers were missed.Comparing to the high-throughput sequencing screen in which a thalassemia carrier rate of 49.5%was identified,only 22.0%was found by traditional methods.This supported that the high-throughput sequencing screen provides a comprehensive assessment of thalassemia screening strategy with high sensitivity and specificity.The transcriptome sequencing and miRNA analyses were employed to explore the mechanism underlining seed dormancy of the precious medicinal herbs Paris polyphylla var.yunnanensis.In this study,we identified 263 conserved miRNAs and 768 novel miRNAs in the seed and seed coat.By the analyses on target prediction and functional annotation of target genes of conservative and novel miRNAs,we found that these miRNAs are directly or indirectly involved in the regulation of cell metabolism and genetic information processing during Paris polyphlla seed dormancy.The results provided the basis for further investigation of breeding and culture of Paris polyphylla.