| With the rapid development of today's animal husbandry,there are many shortcomings in the existing immunopotentiators,it is difficult to achieve satisfactory immune effects,so the study on efficient,secure and affordable new immuno-potentiators has become an urgent problem.On the basis of successful development of astragalus epimedium polysaccharide injection,the production process of astragalus epimedium polysaccharide powder(AEP)was developed and dosage of AEP was screened and its effectiveness was validated,in order to provide the theory evidence of the development of AEP.The experiment was divided into 5 parts.Experiment I.Study on the production process of AEP The dry matter content,minimum spray volume and the adsorption capacity of accessories of AEP was measured.The measurement results of dry matter content of AEP showed:Three parts of dry content in AEP were 12.1%-14.5%,The ratio of dosage accessories were 85.5%-87.9%,so the quality of accessory was 880g.The measurement results of minimum spray volume showed:the ratio of minimum spray volume were 40%-55%,the volume was more than 550ml.The measurement results of adsorption capacity of accessories showedExperiment II.Study on the safety of AEP According to the guiding principles of new veterinary drug safety research,the acute toxicity,subchronic toxicity and safety of target animal chicken of AEP were measured.The results showed that the maximum dosage of AEP was 18000 mg·Kg-1;The mice in all groups behaved short unwell reaction after each lavage and did not appear any untoward reaction,invasion and death during the whole experimental observation.Before administration and on days 1 and 14 after drug withdrawal,the body weight of all mice did not present significant differences.On days 1 and 14 after drug withdrawal,there were not significant differences in the hematological physiological and serous biochemical indexes of all mice and no obvious pathological changes were seen in autopsy and histopathological examination.During the whole experimental observation,the clinical signs of the chickens in all groups were normal,no adverse reaction,invasion and death occurred.Before administration and on days 1 and 7 after drug withdrawal there were not significant differences in chicken body weight of all groups.On day 1 after drug withdrawal,the hematological physiological and serumal biochemical indexes in all chicken were within normal range,no obvious pathological changes were seen in autopsy.These results indicated that the AEP was atoxic,without obvious subchronic toxicity and safe for clinical medication.Experiment?.Study on the dosage screening of AEP 210 14-year-old chickens were randomly assigned into 7 groups and vaccined with ND vaccine except of blank control group,repeated vaccination at 28 days old.when the chicken were vaccined,5 experimental groups were fed with AEP125mg,100mg,75mg,50mg,25mg mixed feed,once a day for three successive days;were not administrated in vaccination control(VC)and BC groups.On days 7,14,21,28 after the first vaccination,use the ?-micromethod to measure the body serum ND-HI antibody tier.The result showed after each administrated,the serum antibody tiers of the groups were fed with AEP are obviously above(VC)and(BC)groups,the 75mg group was the maximal in the first three point time,and arranged second in the fourth point time,indicated the immune effect of AEP were remarkable,75 mg per plume was determined as the optimum dosage.Experiment IV.Clinical trial of resisting chicken immunosuppression of AEP 180 11-day-old chickens were assigned into 6 groups,injected with Cy at 8 mg·ml-1 once a day for 3 successive days except blank control group.On day 14,three groups were fed with AEP for 100 mg,75 mg,50 mg mixed with feed,the pharmic group.was drunk with APS 0.1ml for 3 successive days and the model group and blank group wan not drunk with medicine.The growth of chickens was observed every day after administration.On days 7,14,21,and 28 after the first administration,six chickens were slected randomly from each group for the measurements of the peripheral lymphocyte proliferation and the indexes of immune organs.The results showed that AEP could be able to overcome the Cy-included immunosuppression,enhance the immune response of immunosuppressed chicken.Experiment V Study on the immune-enhancing action of AEP 300 non-immune healthy 14-day-old Roman Roosters were randomly divided into six groups,50 each group.In addition to the control group,all were vaccinated with NDV IVvaccine with the by the way of nasal inhalation and eye droppings,At the same time of the vaccination,the chickens in drug groups were mixed feeding high(100 mg),middle(75 mg),low(50 mg)dose of AEP.The drug control group each drinking with 0.1 ml of Astragalus polysaccharide oral liquid,1 times a day for 3 days;the immune control group and the blank control group were not given drug.On days 7,14,21 and 28 after the first vaccination,selected 6 wing randomly in each group to carry out the venous blood sampling,detected the serum ND-HI antibody titer;selected 4 wing randomly in each group to carry out cardiac blood sampling,detected the proliferation of lymphocytes by MTT;selected 6 wing randomly,weighing,calculating the average body weight.The results showed that in all time points after immunization,the AEP group of the serum antibody titer,cell As70 values and average weight were much higher or were significantly higher than those in the immune group middle dose of AEP of antibody titer values,cell A570 values were the highest,and significantly higher than the VC group the average weight and high dose group alternately heaviest,which was significantly heavier than the VC group.It shows that AEP can significantly enhance the immune effect of NDV IVvaccine,and growth promoting effect,middle dose group has the strongest effect,it is recommended for clinical application dose. |
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