Screening Of Transgenic Soybean Germplasm To Salt Tolerant

Soybean is a moderately salt-tolerant crop.From germination to grain maturation,various stages are affected by various abiotic factors.Salinization is attracting more and more attention.Studies have been found that transgenic soybean can effectively improve soybean salt tolerance,so as to ensure crop yield in recent years.In this paper,83 soybean Material provided by the Jilin Academy of Agricultural Sciences were used as the materials.The transgenic materials were further selected through preliminary screening.Different concentrations of NaCl(0,100,150,200,250 mmol/L)were applied in the bud stage of soybean to study the effect of salt stress on the germination characteristics of soybean seeds,and the ratio of soil and vermiculite was 1:3 in soybean seedlings and flowering stages,250 mmol/L NaCl was applied to investigate the effects of salt stress on the growth,osmoregulation,antioxidant system and photosynthesis of soybean seedlings.The yield was measured at maturity to determine the resistance to salt soybean material.The aim is to provide theoretical basis for agricultural production and soybean test materials.The results show:(1)Under the condition of 250 mmol/L NaCl stress,83 transgenic soybean materials provided by the Jilin Academy of Agricultural Sciences were initially screened,fresh weight,dry weight,MDA content and relative conductivity of seedlings were measured at the seedling stage.In total,11 transgenic Material were screened out based on those four indexes: 1)NPK1 gene: 2;2)BADH gene: 2;3)HAL1 gene: 7;(2)Under different salt stress conditions(0,50 mmol/L,100 mmol/L,150 mmol/L,200 mmol/L,250 mmol/L),germination germination rate,root length and root number of transgenic material potential were significantly higher than those of the control treatment,With the gradual increase of salt concentration,the germination index of soybean Material gradually decreased.When the salt concentration reached above 200 mmol/L,the inhibition of germination index of soybean Material was very significant.(3)In the seedling and flowering period of soybean,under the stress of 250 mmol/L NaCl stress,the inhibition of salt stress on soybean seedlings can be alleviated after the salt tolerance gene is transferred into the receptor variety.At the same time,the decline in fresh weight,dry weight,plant height,and leaf area at the seedling stage was greater than that at the flowering period.The sensitivity of the seedling stage to salt stress was higher than that of the flowering period.(4)At the seedling and flowering period of soybean,the proline content,soluble protein content,SOD activity,POD activity,chlorophyll content,Pn,Gs and Tr of the transgenic soybean Material were significantly higher than those soybean Material under the stress of 250 mmol/L NaCl concentration,but the content of MDA and relative conductivity is lower than the respective receptor Material,indicating that transgenic Material can effectively regulate intracellular osmotic adjustment substances,reduce osmotic potential,maintain the stability of cell membranes,and increase the antioxidant enzyme activity of seedlings.Relieve the damage to the photosynthetic system of seedlings,and enhance the salt tolerance of the plant.At the same time,the ability of soybean seedlings to regulate salt stress in the flowering period was higher than that at seedling stage.(5)The plant height,number of branches and number of main stem sections of the transgenic Material were higher than those of their respective receptor Material comparison in the saline pool.The number of single plant per plant,grain number per plant,grain weight per plant,100-grain weight and yield were higher in the transgenic Material,indicating that the transfer of salt-tolerant genes into recipient Material can regulate the components of yield and thus promote the formation of yield.