Preliminary Establishment Of A Rapid Propagation System Of Kolopanax Septemlobus

Kolopanax septemlobus(Thunb.)Koidz.which belongs to Araliaceae,Kalopanax Miq,is one of the species of national second-class rare and endangered plants.It has wide application value in ornamental,medicinal,timber and edible areas.Wild resources are scarce,the traditional propagation methods are mainly due to the limited seed resources,seed dormancy,and are difficulty in sowing and rooting.Therefore,in this experiment,callus was used as test material to re-differentiate and produce adventitious buds,then adventitious buds were proliferated and cultured,and roots were induced to establish a complete and efficient tissue culture rapid propagation system of Kolopanax septemlobus.The induction effect of callus was significantly different which using the different culture mediums.The experiment showed that,different kinds of basic medium has a certain effect on the callus induction,the highest induction rate reached 89.3%when it usd MS as basic culture medium.The fresh weight was 539.4 mg,which was significantly better than other four mediums.The kinds of factors in the process of redifferentiation on callus of Kalopanax septemlobus were filtrated to obtain a suitable culture medium.When KT was 2.0 mg·L-1 and NAA was 0.10 mg·L-1,the hormone combinations would be better.Adding 30 grams of sucrose per medium,the effect of callus re differentiation was the best,and produced 12.6 adventitious buds,the differentiation rate was 94.4%,the average bud was 1.3 cm.The growth of adventitious buds tended to increase firstly and then decrease with the increase of culturing time.The shoot growth was vigorous at 40 days,with 12.0 adventitious buds,the rate of differentiation was 93.0%and the average shoot bud length was 1.2 cm.The type and concentration of different cytokinins on adventitious bud of Kalopanax septemlobus subculture had obvious effect The fresh weight and dry weight,reached 2307.1 mg and 205.4 mg,which it reached the highest level when the concentration of BA was 1.5 mg·L-1.And the multiplication coefficient reached 9.1,which was better than other concentration treatments.The cluster bud density is moderate,and the single bud can be cut in the aseptic operation.The growth of plant was best at the concentration of NAA was 0.10 mg L-1 and the proliferation was better than other concentrations of the same species,and the proliferation coefficient was 9.6.Therefore,BA1.5 mg·L-1 and NAA 0.10 mg·L-1 was the best to promote adventitious buds combination of plant hormone concentration subculture.The results showed that adding 1 grams of PVP to each litre medium could effectively reduce the browning of plants during subculture,the browning ring decreased significantly.The anti browning effect is better than Na2S2O3 and ascorbic acid Vc that are two kinds of anti browning agents.The rooting rate of Kalopanax septemlobus during rooting culture was 333%when IBA was 2.0 mg L-1.A few roots occurred,differentiated less and grew weaker,and the upper leaves gradually withered Adventitious root proliferation experiment confirmed that BA and IBA hormone is the best combination of adventitious root and it produced the more stout adventitious roots.When the concentration of BA was 0.3 mg·L-1,the adventitious root was white and villous,the growth was well.The multiplication of adventitious root was 11.6 when the concentration of IBA was 2.0 mg·L-1.