Studies On The Stem Segment Tissue Culture Of Xanthoceras Sorbifolia

This paper used lignified stem segments of Xanthoceras sorbifolia as explants, researching on the sterilization methods of the stem segment,the induction of axillary buds sprouting,the non embryogenic callus induction, the embryogenic callus induction.the adventitious buds induction、proliferation、rooting, and weak seedling rejuvenation and so on, establishinging the system of the stem segment tissue culture of Xanthoceras sorbifolia through the experiment.he results of the study show that:1、For the stem segments the most suitable sterilization method should be 75% alcohol 30s+5% NaCIO 8min+0.1%HgCl2 10min (added 2 dro of Twain-20ps), the survival rate was 45%,the infection rate was 31.67%, the mortality rate was 23.33%.2、Suitable medium of inducing germination of axillary buds was MS+6-BA1. 0mg·L-1+IAA2.0mg·L-1,the axillary buds germination rate was 96.30%, after 7day-s’culturing, the axillary buds length up to lcm, robust growth.3、Suitable medium to induce non embryogenic callus was B5+1.0mg·L-16-B A+0.5mg·L-1IAA+3.0g·L-1sucrose+8g·L-1agar,the non embryogenic callus grew fast a nd more, the induction rate was 84.00%; the induction of the embryogenic callus should choose the suspension medium B5+0.5mg·L-16-BA+0.5 mg·L-1NAA+0.5 m g·L-12,4-D+ sucrose 20 g·L-1+hydrolyzed milk protein 500mg·L-1, the pH value was5.4, the callus browned little,differentiated to the similar white root substance, was more easy to induce adventitious buds,the better effect than the solid mediu-m.4、Using the embryogenic callus to induce the adventitious buds, the most suitable culture medium was MS+0.1mg·L-16-BA+1.5mg·L-1IAA+ 30 g·L-1 sucrose+8 g·L-1 agar,the adventitious buds induction rate was 85.56%, the adventitious buds grew well.5、The most suitable medium for adventitious buds proliferation was MS+0.1 mg·L-16-BA+0.5mg·L-1IAA+2.0mg·L-1GA+30g·L-1 sucrose+8g·L-1agar, the proliferation number was up to 5.72, the buds height was up to 4.89cm, they grew best.6、The most suitable medium of adventitious buds rooting was WPM+2.0mg·L-1IBA+2.0mg·L-1ABT, the pH value was 5.7, the rooting rate was 86.67%, the see dling rate was 30.00%, the average rooting number was 2.78, the average rooting length was 58.92mm.7、The medium for strengthening the adventitious buds was WPM+2.0mg·L-1I BA+2.0mg·L-1ABT+0.5% active carbon, the adventitious buds lignification strength ened, helping to alleviate the part on the ground dry death after rooting,improving the tissue culture seedlings survival rate, the seedling rate was 69.41%;for the p1 ants whose part on the ground grew not well, when the root length was up to 10 mm, the most suitable medium for weak seedings rejuvenation was MS+0.2mg·L-1 6-BA+0.5mg·L-1IAA,the part on the ground grew vigorously well, leaves increased, taproot elongated and sprouted;for the plants whose part on the ground were dead after rooting,the most suitable medium for inducing the adventitious buds was W PM+0.2mg·L-16-BA+0.5mg·L-1IAA,there were adventitious buds sprouting on the r-oots again, the adventitious buds grew well and gradually grew into complete pla-nts.the budding rate was 83.33%.