Study On Pretreatment Of Corn Stalk And Its Use As Cultivation Medium For Edible Fungi

The demand of edible fungi is increasing year by year,but the main production materials cottonseed hull and sawdust are decreasing year by year,and the production cost of edible fungi is increasing,so it is necessary to find effective substitute materials.China is a big agricultural country with abundant straw resources.Applying straw into edible fungi cultivation can not only reduce the production cost,but also relieve environmental pollution and realize the utilization of straw resources.In this paper,the pretreatment process of corn stalk(ammonia treatment+ biological detoxification)and the effects of straw treatmenton edible fungi cultivation were studied with the aim of promoting corn straw as the cultivation medium of edible fungi.the following results were obtained:(1)Lignin derivatives have inhibitory effect on mycelial growth.The biomass of Volvanriella volvacea mycelium in liquid medium containing 0.1-0.5 g / l syringaldehyde,vanillin and 4-hydroxybenzaldehyde decreased by 7.46 %-51 %,28.86 %-94.44 %,38.21 %-97.70 % respectively.The biomass of Pleurotus ostreatus mycelium decreased by 15.17 %-78.87 %,4.17 %-98.87 % and 8.64 %-99.32 % respectively.The biomass of Lentinus edodes mycelium decreased by 8 %-84 %,20.52 %-98.30 % and 11.26 %-98.09 % respectively(2)Lignin derivatives can reduce the activity of cellulase and xylanase.Adding 0.1-0.5 g / l syringaldehyde,vanillin and 4-hydroxybenzaldehyde into the reaction substrate,the cellulase activity decreased by 7.84 %-19.68 %,1.92 %-39.82 % and 1.15 %-19.25 % respectively.Xylanase activity decreased by 62.46 %-71.19 %,61.49 %-70.29 % and 57.58 %-65.52 % respectively.(3)Inoculating 1 % Acinetobacter calcoaceticus to culture for 2 d can reduce syringaldehyde content to 0.11 g / l,vanillin content to 0.10 g / l,and 4-hydroxybenzaldehyde content to 0.01 g / L.(4)Pretreatment can effectively improve the reducing sugar content of straw enzymolysis.Corn stalk was treated with 3 % ammonia at room temperature for 4 d,then inoculated with Acinetobacter calcoaceticus,and fermented for 2 d.The reducing sugar content of 2-10 mesh stalk was increased from 0.332 g/g to 0.433 g/g after enzymolysis.The reducing sugar content of 10-35 mesh straw after enzymatic hydrolysis increased from 0.338 g/g to 0.461 g/g.(5)Pretreatment of corn stalk can improve the planting benefit of Volvanriella volvacea,Pleurotus ostreatus and Lentinus edodes.Compared with the control group,the addition of ammonia-treated fermented corn straw shortened the planting time of Volvanriella volvacea by 1.3 d,increased the mycelial growth rate by 1.13 mm/d,shortened the bottle filling time by 3.4 d,improved the biological efficiency by 74 %,increased the crude protein content by 9.30 %,and increased the polysaccharide content by 48.10 %.There was no significant influence on the planting time and full bottle time of Pleurotus ostreatus,the mycelial growth rate increased by 0.32 mm/d,the biological efficiency increased by 7.54 %,the crude protein content increased by 25.64 %,and the polysaccharide content increased by 22.79 %.There was no significant influence on the colonization time,mycelial growth rate,bottle filling time,biological efficiency and crude protein and polysaccharide content of Lentinus edodes.