| Anthurium is an important potted and cut flower in China.Developing highly blight resistant and colourful varieties are the main objects for anthurium breeding,which was hampered by the long periods of time and low efficiency.By contrast,molecular assisted breeding has attracted wide attention for its advantages of high efficiency and precision.In this study,199 F1 progeny randomly selected from 'Acropolis'(blight resistant,white spathe)×'Alabama'(blight susceptible,red spathe)were used to develop SNPs and construct the high density genetic map through high throughput SLAF-seq.And then,QTLs mapping for blight resistance and spathe colour were performed.The key results as followed:(1)SLAF-seq developed 1,215.07M reads,with an average Q30 of 90.09%and an average GC content of 42.09%.A total of 537,356 high quality SLAF tags were developed.Among which,the female parent obtained 192,013 SLAF tags with an average sequencing depth of 34.33 ×.The male parent obtained a total of 194,587 SLAF tags with an average sequencing depth of 35.46 X.The progeny produced a total number of SLAF tags of 150,756 with an average sequencing depth of 13.43 X.There are a total of 169,020 polymorphic SLAF tags and a 49.08%polymorphism.(2)Genetic maps were constructed by using the "double pseudotest cross" strategy.The female map included 2,326.85 cM length,4,440 markers and the average plot distance of 0.65 cM.,the male map with a total length of 1,896.74 cM,included 4,287 markers and the average plot distance was 0.64 cM.For the integrating genetic map,7,065 markers were distributed on 15 linkage groups,and covered 2,246.36 cM with an average plot distance of 0.37 cM.The segregation mark was 272,which accounted for 3.85%of the total number of marks.The markers shows the completeness of 99.87%.(3)For blight resistance,in vitro leaf disc inoculation was used to identify the resistance of F1 progeny and its parents.Resistance was determined based on the time taken for lesion to cover the entire leaf disc(TLC),which ranged from 2 to 25 days in F1 population and the resistance showed continuous variation.The normal distribution pattern is present in the mapping population.Three QTLs related to resistance were detected by MapQTL6.0.The LOD ranged from 2.02 to 2.27 and the contribution rate was 4.1%to 5.3%.Twenty-four resistance-related QTLs were detected using GACD1.0.LOD values range from 2.0062 to 3.1369.The contribution rate is between 0.3462%and 3.9058%.The co-located marker is Marker17072.(4)The flower color phenotypes were investigated by visual observation and colorimeter measurements.The visual separation of F1 populations was red,white,pink,and orange;the L*a*b*and C*in the colorimeter measurements were significantly correlated with the eye observations.After a normal distribution test,L*a*b*,C*,h value and macroscopic observations all showed a continuous variation,which basically accorded with a normal distribution.By the K-S test,the P values of the L*,a*,and C*values are all greater than 0.05 and are applicable for QTL mapping.(5)The QTLs are mapped to linkage map by the brightness L*,the red and green values a*,the chroma C*,and the L*,the red and green values a*,the chroma C*values obtained by the MapQTL 6.0.The three QTLs mainly concentrated on the LG06,LG07,and the LG12.The maximum LOD is 20.82 and the contribution rate ranges from 7.5%to 39.5%.Using the GACD1.0 composite interval mapping method,12 QTLs were mapped.5 QTLs were mapped by L*values.The LOD values were among 3.307-14.3589,and the contribution rate range from 0.735%to 5.1759%.Six QTLs were mapped by a*values,with LOD values ranging from 3.2913 to 10.2753.The contribution rate was among 0.84%-6.9582%.Six QTLs were mapped by C*values,with LOD values ranging from 3.0924 to 4.2314,with a contribution rate from 1.335%to 8.289%. |
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