| Anthurium andraeanum, a kind of famous and precious flower introduced to China in recent years, has become the favorite of most people for its peculiar appearance, long flurescense and rich colors. However, the development ofAnthurium andraeanum sector in China was still far from booming due to the backward traditional method of propagation, which was limited to special seasons and of low propagation rates. Tissue culture, applied to rapid propagation of large scale in flower industry, was considered a possible way for Anthurium andraeanum. Unfortunately, many former researchers had tried hard but failed in it, especially in plantlets hardening, transplanting and disease controlling. After systematic researches on related factors such as explants, basic media, hormones, carbon resources, pH, light and additives, the traditional techniques of Large-scaled tissue culture of Anthurium andraeanum in promoting calli induction and buds differentiation were optimized successfully. Survival rate of transplanting was improved through chemical soaking and adaptive training before transplanting, and growth speed was accelerated through discussing the influences of substance, illumination, etc. At last, the pathogenic bacterium of blight was identified initially and was inhibited by streptomycin. The results as flowing:1. Improvement on tissue culture techniques of Anthurium andraeanumFor calli inducing, a combination of BA-6 with 2.4-D was more effective than single of them, enhancing inducing rates by about 8% and 30% respectively. The rate of calli inducing also rose up to 100% hi the improved medium with reduced N to 250mg/l in the form of NH4NO3 and KNO3, 15% more than that of previous researches. Different forms of hydrocarbon resources also led to different calli inducing rates and calli qualities. Calli inducing rate could reach 100% in 30g/l sucrose or glucose, 13% higher than in maltose, and the quality of induced calli hi glucose was much better than hi others. Taking leaves and petioles of sterile plantlets as explants, the inducement rates of different varieties were more than 97%. Taking materials from pot plantlets, the petioles of frondesces and blondes of ripe leaves were suitable to induce calli. Modified MS+ BA0.2(0.5orl.O) +2.4-D0.1 +glucose30g/l was proved to be the proper medium for calli induction.Comparatively higher concentration of BA and low concentration of NAA had positive effects on buds differentiation, and it could be up to 96.1% by glucose as carbon resource. Modified MS+BA2.5+NAA0.5+glucose30g/l was the proper medium for buds differentiation, the quality of buds was better, differentiation rate was higher and quantity was more.Axillary buds, another kind of ideal explant, multiplied by 5.6 times in modifiedMS+BA1.0+NAA0.1.Basic medium had effects on subculture. Rates of increase on fresh weights of test tube plantlets on modified MS and MS were different extremely remarkably. The culture media containing coconut milk promoted the growth far more effectively than non-coconut milk ones. Strong light was also a stimulus for the growth of test tube plantlets and in the light of 2500Lx to 5500Lx, the increase rates of fresh weight was much higher than those in weaker light. The modified MS+BA(KT)+NAA0.05 +glucose30g/l +CM100g/l was proved to be proper medium for subculture, in which the test tube plantlets were much stronger and 3-5 roots could be induced from it. This kind of plantlets could be transplanted directly.2. Studies on factors to transplanting survival rate and growthThe Carbendazin-sulfar was superior to KMnO4 in dipping treatment of test tube plantlets against infection before transplantation. 20 minutes such treatment m 0.2% of Carbendazin-sulfar made the survival rate up to 91.1%, 12% higher than that of KMnO4.Exposed to the outside environment for 5 days by uncovering plastic mantle test tube plantlets could survive by 86% and more than 7 days exposure led to 98% survival. The test tube plantlets with 3 or more roots often grew better. According to increment of...
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