A Preliminary Study On The Different Rapid Propagation Systems Of Cerasus Discoidea And Cerasus Conradina

Cerasus spp is a famous woody ornamental flower.In recent years,the flower cultivation is in full swing and the seedlings are in short supply.The species of Cerasus spp were more studied in weet cherry,sour cherry and other varieties with high edible value such as Prunus spp and less research on that with high ornamental value by tissue culture.In our study,we selected 20 new strains of domestic Cerasus spp from many provinces,including four kinds of homemade Cerasus spp which also wild ornamental plants are Cerasus schneideriana,Cerasus conradinae,Cerasus discoidea and Cerasus dielsiana,respectively.Using a half lignification twigs in its born that year as basic material,from establishing the sterile system,successive transfer culture to root induction in a rapid propagation technology system research,and analysis the key problem were high pollution rate and low axillary bud induction rate which did not solve in the process of cultivating until now,in order to realize the theoretical foundation of the domestic industrialization of Cerasus spp seedling to provide professional and technical support.The main results are as follows:Fristly,the aseptic system in domestic Cerasus spp was built.Sanitized respectively by Hg Cl2,secondary mercury disinfection and Na Cl O in explant culture and sieved the better method is that 70% ethanol was soaked in the spring stem for 30 s,and then 0.1% Hg Cl2 was soaked 2.5min in 0.01 ml can control the pollution rate in 19.17%.70% ethanol is soaked for 30 s in summer;the best treatment time was 3min by soaking with Hg Cl2 so that it can control the pollution rate in 21.67%.Secondly,the axillary buds of domestic Cerasus spp.Using orthogonal design and sieved the better scheme of the medium for induction axillary bud of C.discoidea is 1/2MS+0.6mg/L6-BA+0.1mg/L NAA+1.0 mg/L KT and its germination rate is 51.67%.Then,for C.conradinae,the axillary induction medium is 1/2MS+0.6mg/L 6-BA+0.1mg/L NAA+1.0 mg/L KT and its germination rate is 45.00%.Thirdly,the proliferation culture of domestic Cerasus spp.Using orthogonal design and sieved the better scheme of the medium for proliferation culture of C.discoidea is WPM+1.5mg/L6-BA+0.15mg/L NAA and its multiplication is 6.3.Then,for C.conradinae,the medium for proliferation culture is WPM+0.5mg/L 6-BA+0.45mg/L NAA and its multiplication is 11.2.Fourthly,orthogonal design was applied to screen out the best suitable medium for Cerasus discoidea and Cerasus conradinae is WPM+0.2 mg/L6-BA+0.03 mg/L NAA.