| Flowering cherry is a famous woody ornamental plant,which is a general name of the Prunus Subgenus Cerasus plant of Rosaceae.The variety resources of flowering cherry are very rich at home and abroad.Prunus Subgenus Cerasus plant are more and more popular for their high ornamental value,economic value and ecological value.With the extensive application of ornamental flowering cherry in gardens,traditional grafting and seeding are far from meeting the market demand.Prunus Subgenus Cerasus×incisa‘Gotenba-zakura' is a good ornamental safflower varieties,which is a kind of excellent woody material for studying tissue culture and cutting propagation,The exploration of the methods of rapid tissue culture micropropagation and cutting propagation of Prunus Subgenus Cerasus×incisa‘Gotenba-zakura'is beneficial to provide an effective way for the rapid propagation and industrial production of flowering cherry,so it has great practical significance for the propagation of flowering cherry.The main results are as follows:1.Tissue culture and propagation by stem segments with buds:(1)The appropriate disinfection and sterilization method is 75% alcohol rinsing for 30 s and 2% Na Cl O treatment for 2min,then the pollution rate was 46.7% and the survival rate was 43%.(2)The optimum medium for lateral bud induction is MS+6-BA3.0mg/L+NAA0.2mg/L.The rate of lateral bud induction reached 85%,and the average number of lateral bud induction was 1.3.(3)The optimum medium for proliferation culture is MS+6-BA3.0mg/L+2,4-D0.6mg/L+GA1.0mg/L.The best medium to promote the growth of seedlings is MS+6-BA2.0mg/L+2,4-D0.4mg/L+ GA0.5mg/L.It can be used alternately with the anti-browning medium added with activated carbon 1.0g/L,and the alternating period is one week,which can effectively reduce the Browning rate.(4)The optimum medium for rooting is MS+ABT 8g/L+sucrose 60g/L.The rooting rate was 100% and the average number of rooting was up to 17 and the induced root system was robust..(5)The appropriate time of smelting plants is 5 days,The transplanting medium is vermiculite and peat volume ratio 1:1.The survival rate was 90% and the transplanted seedlings grew well.2.Study on callus regeneration:(1)The best disinfection and sterilization method is 75% alcohol rinsing for 30 s and 1% Na Cl O treatment for 30 s.The contamination rate was controlled at about 15% and the survival rate was 58%.(2)To induce callus,leaves on the upper part of annual branches should be selected as explants.The optimal culture method was inoculated in the medium of 1/2MS+6-BA2.0mg/L+NAA0.3mg/L+2,4-D1.5mg/L,followed by dark culture for 5 days and then culture under light conditions.The induction rate was the highest,and the callus morphology was dense and granular,with green color and red edge.(3)The medium suitable for callus proliferation is 1/2MS+6-BA0.5mg/L+NAA0.05mg/L+AC0.5g/L,The proliferation factor of callus reached about 3.5 after 21 days.Anti-browning agent can significantly reduce the Browning rate of callus,the color of the proliferated callus is green,the edge is red,and the texture is dense granular.(4)The best medium for callus differentiation is:1/2MS+6-BA1.0mg/L+IBA0.03mg/L+GA1.5mg/L+sucrose60g/L,The differentiation rate of adventitious buds was 6.67%.3.Cutting propagation by stem segments with buds:The results showed that the ability to affect the rooting of Prunus Subgenus Cerasus×incisa‘Gotenba-zakura' cuttings was that the species of plant growth regulator was greater than the cutting base than the plant growth regulator concentration.The best cutting scheme is to soak the cuttings in ABT solution of 400mg/L for 30 min and then planted them into vermiculite.The cutting rooting rate is the highest,which can reach more than 75%.The rooting number was large and the length was long,and the roots were evenly distributed. |
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