| In this paper,genetic diversity analysis and evaluation of drought resistance at the bud stage for 226 mung bean resources were carried out,and22 pairs of primers were used for genetic diversity assessment and cluster analysis and population structure analysis.The following are the main results:1.Drought resistance evaluation of mung bean at the bud stage:226 mungbean varieties were treated with 15% PEG6000 hypertonic solution to simulate the drought stress.The germination rate,germination potential,germination index,vigor index and relative root length were measured.The results showed that 1 high-resistance germplasm,5 resistant germplasm,209 moderately resistant germplasm,11 sensitive germplasm,and 0 sensitive germplasm were obtained at 15% PEG concentration.Among them,these indicators for evaluating the drought resistance of mung bean were positively correlated with each other,and the germination potential and the germination index were significantly positively correlated among them.2.Genetic diversity of agronomic traits in mung bean:By investigating the 11 agronomic traits of 226 mung bean germplasm,the genetic variability of agronomic traits was evaluated.The results showed that the highest genetic diversity index was the growth habit(0.12)in quality traits,and the highest genetic diversity index was number of stems(2.07)in the quantitative traits.UPGMA clustering classifies 226 materials into 6categories.The results show that there is no corresponding relationship between the clustering results and the geographical origin of the germplasm resources.The genetic diversity of mung bean materials in 10 different geographical areas has significant genetic variation,among which Shanxi has the highest genetic diversity index(1.58),and Beijing(1.21),Hebei(1.18)and northeast China(1.18)have higher genetic diversity indices,while Neimenggu and Asian vegetable center are the lowest;Shanxi has the largestnumber of materials,and its characteristics in terms of plant height,length,and growth habits can be used for related genetic research and new gene discovery;UPGMA clustering will divide these 10 different geographical regions into two categories.Asian vegetable center is classified as one,and the remaining nine groups are classified as one.This has a certain relationship with its geographical origin.3.Analysis of genetic diversity of mung bean based on SSR markers:In this study,22 pairs of SSR primers were used to analyze the genetic diversity of mung bean populations from 10 different geographical sources(226 germplasms).The results showed that a total of 87 alleles were detected,and the average number of alleles(NA)was 3.95,and the average effective number of alleles(NE)was 2.73,and the average observation heterozygosity(Ho)was 0.0907,and the average expected heterozygosity(He)was 0.6176,and the average Shannon Index(I)was 1.10,and the average polymorphism information content(PIC)was 0.5527.It was found that the values of NE,He,I and PIC in Shanxi Province had the highest values in all groups,indicating that the mungbean resources in Shanxi had the most abundant genetic diversity;followed by Beijing and Hebei.The NE,He,I and PIC values in the Asian vegetable center,Neimenggu and Chongqing districts were all low,indicating that the genetic diversity of mungbean germplasm resources in these three regions was low.Based on the results of the structure software,226 participants were divided into 6 categories,which were consistent with the clustering results of 226 participants who were clustered into 2 categories based on Popgene software;the clustering results based on Popgene software divided 10 different area groups into four major categories.The closer the geographical sources are,the closer the genetic relationship is. |
PDF Full Text Request