Using Edible Fungus Residue To Produce Feed Protein

Edible fungus residue(EFR)is a waste material of culture medium after cultivation of edible fungus,which itself contains a higher crude protein content,but the content of crude fiber is also high.If it is directly used as an animal feed,the digestibility is low,the palatability is poor,and the nutritional value is not very high.In this dissertation,the EFR was used as a research object,and a comprehensive study was conducted on the technology of pretreatment and solid state fermentation of the raw materials,and then,the factory is designed.The content of the paper is divided into the following three parts:In the first part,the effects of chemical methods,physical methods and biological methods on the removal rate of lignin and cellulose degradation in raw materials were investigated.The results showed that the use of chemical reagent NaSO3,NaClO and ureophil for thepretreatment of raw materials,lignin removal rate is low,the use of alkaline solution and alkaline hydrogen peroxide solution treatment of raw materials can be better to lignin removal,the highest removal rate is 71.87%,but at the same time,the loss of crude protein is severe and the highest loss rate is 84%.Therefore,the chemical method is not suitable for the pretreatment of the raw materials.The instrument of physical method is the plant fiber processor.In the wet milling treatment,the nutrient content and soluble metal ion content before and after treatment did not change significantly,and all had a typical lignocellulose structure.However,by observing the SEM image,the raw material structure became loose.And detected by XRD,the cellulose crystallinity of the treated raw materials was reduced from 31.18%to 30.97%.In the process of biological treatment,a strain of Bacillus subtilis that can secrete cellulase by fermentation was first screened,and the secreted CMCase activity was 83.94 ?mol/(mL min),the filter paper activity was 98.81?mol/(mL min),and the secreted amylase activity was detected to be 3.968U/mL,the protease activity was 60.28U/mL,mixed fermentation with 2.5%Aspergillus niger,the highest cellulose degradation rate was 30.96%.Therefore,the best pretreatment method is using a plant fiber disintegrator to wet milling the raw material first,and then,mixed inoculation of Aspergillus niger and Bacillus subtilis to ferment for the purpose of reducing the content of lignocellulose.In the second part,the optimal fermentation conditions for increasing the crude protein content were investigated.The results are as follows:Firstly,a strain of yeast with good growth status and high conversion rate of ammonia nitrogen and reducing sugar was screened.The conversion rate of ammonia nitrogen was 89.21%and the reducing sugar conversion rate was 96.82%.The inoculation amount is 10%,t=3d,T=30?,pH=6,solid-liquid ratio=1:2,C/N=30:1,adding bran and DDGS at a ratio of 5%each,the crude protein content of fermentation product can reach 27.02%,which is 1.66 times higher than that of raw material,and the pepsin digestibility is 43.77%,which is 33.03%higher than 29.31%before fermentation.The third part combines the results obtained by the laboratory to carry out industrialization amplification,and determines the technical plan for the production of protein feed by fermenting 30,000 tons of edible fungus residues per year.Finally,the investment annual profit is calculated to be 1,301.04 million yuan,and the profit rate is calculated.It is 86.74%and the payback period is 2.15 years.