Development Of Microbial Copper And Its Biological Efficiency Evaluation For Finish Pigs

The purpose of this study is to screen out the microorganism with enrichment and absorption conversion of inorganic copper sulfate as microorganism copper,in order to develop a highly efficient green microorganism copper additive.The contents include the screening of microorganism copper rich bacteria and the optimization of microbial enrichment and conversion of Inorganic Copper to the culture conditions of microbial copper conversion;the comparative metabolism of microbial copper and Inorganic Copper feeding chickens is the following two parts.Experiment 1:Screening of copper rich microorganisms and optimization of culture conditionsA high efficient copper rich bacteria was isolated to convert inorganic copper tomicrobial copper.The microbial transformation of inorganic copper enrichment microbial transformation rate of copper as an indicator,the flame atomic absorption determination of copper content in cell spectrophotometry test by plate dilution method from high copper in the soil was isolated from a strain of Inorganic Copper efficient conversion of fungus FT7,and 26S rDNA identified with inorganic copper into microbial transformation rate of copper as the standard,the L₉?3⁴?orthogonal design of the temperature,pH,temperature,inoculation and incubation time 4 factors were optimized.The results showed that fungus FT7 was Aspergillus Niger,and its best enrichment and transformation conditions were temperature 29.5?C,pH5,inoculation amount 4%and time 5.5 d,and the highest conversion rate was 87.65%.Aspergillus niger is a feeding microorganism,and the microbial copper obtained from the conversion of inorganic copper can be used as a new feed additive in the feed.Experiment 2:comparative study on metabolism of Aspergillus niger andinorganic copper copper feeding pigsThe aims of experiment is to compare the apparent digestibility and metabolic rate of copper,zinc and feed nutrients between organic Aspergillus niger copper and inorganic copper sulphate for finishing pigs.Three heads of 60kg fattening ternary hybrids were selected for metabolic experiments.The trial was divided into two phases and each period was 6 days.The first phase was to measure the inorganic copper sulfate diet that was the sole copper additive for the diet.The second phase was to determine the copper diet of organic Aspergillus niger that was the sole source of copper additives for the diet.Tthe two phases was the same diet except for the type of additive copper.The level of copper was 60mg/Kg both two diet.The results showed that there was no significant difference in the apparent digestibility of dry matter,crude ash,crude fat and apparent metabolic rate of crude protein and energy in the finisher diets for the two different types of copper additives?P>0.05?,and for crude protein and energy.There was no significant difference in metabolic rate?P>0.05?.However,the addition of Aspergillus niger significantly increased the apparent digestibility of dietary Cu and Zn?p<0.05?,and significantly increased the apparent metabolic rate of Zn.The apparent digestibility of Cu and Zn increased 6.82%and 3.5%compared to CuSO₄ and the apparent metabolic rate of zinc increased by 3.93%.Aspergillus niger can replace the copper sulphate feeding pig without affecting the pig's digestion and absorption of dietary nutrients,thereby reducing the emission of fecal copper and fecal zinc.It can be used as a new green trace element additive.