Study On The Distribution Of Local Neurotransmitter In Blue Fox Testis At Different Breeding Period

Seasonal breeding is an important way for animals to adapt to the reproduction of their environment.The factors that affect the reproductive status of animals include light,temperature and food,and the most important regulatory factor of which is light.Due to the seasons effect on biological activities,many animals have relatively fixed breeding cycles(breeding period and breeding period)for estrus,mating and brooding.Blue fox was a typical seasonal breeding economic animal whose testicular tissue neuromodulation and hormone secretion jointly regulate their reproductive physiological functions.Sertoli cells and interstitial cells participate in the regulation of hypothalamic-pituitary-testicular axis neuroendocrine regulation and blood-Testis barrier formation mechanism in order to maintain the testicular spermatogenesis and other functions.Testicular nerve distribution is broad and selective.This study was conducted to characterize the histochemical characteristics and distribution of neuromodulation proteins: PGP9.5,NPY,ChAT and DBH in Blue fox testis during different breeding seasons by H.E dyeing and immunohistochemical SP technique combined with Image-Pro Plus(IPP)statistical methods.The results showed that PGP9.5 and NPY were significantly higher in the blue fox testis during the reproductive stage than those in the reproductive stage,and in small blood vessel wall positive strongly,suggesting that peptide neurotransmitter may be mainly involved in the regulation of testicular function through microcirculation of small blood vessels;the positive expression of ChAT in the reproductive phase mainly seen in Ley cells,and its expressed in reproductive period higher than the reproductive interval,it may involved in regulation testosterone secretion.Also DBH may be involved in the regulation of reproductive function,the mechanism remains to be further analyzed.In conclusion,the different distribution of neurotransmitters in blue fox testis at different breeding seasons indicating that the neural regulation plays an important role on the reproductive function of male seasonal breeding control.