Diagnosis And Studies On The Pathology Of The Peste Des Petits Ruminants Of Sheep

Peste des petits ruminants(PPR)is the infectious diseases of acute,febrile and highly contact by PPRV caused,mainly infect goats and sheep and other small ruminants.The disease is A class of animal epidemics in the OIE and in China.Since July 2007 peste des petits ruminants(PPR)has erupted in many regions in our country.At present,the disease has enormous threat to sheep breeding in China,and also seriously affect the meat hygiene safety in China.It was be used that the pathologic autopsy,histochemical technique,image analysis technology,transmission electron microscopy technology in this research.The 7 cases of ill sheep to be suspected PPR from Ningxia Wuzhong were studied by the pathology and diagnosis system.The results showed that:(1)According to the PPRV N gene fragment RT-PCR amplification detection results,it is proved that the multiple samples of 7 cases of disease sheep contain PPRV.At the same time,the sheep disease of the field is also proved to be peste des petits ruminants(PPR).(2)Through the PPRV N gene RT-PCR amplification detected the consistent with the expected purpose gene size banding,and other related pathogenic RT-PCR detection is negative results,proved PPRV N protein high specificity and sensitivity in the diagnosis of small ruminants.(3)The pathological observation showed that the sheep small ruminants(PPR)disease sheep has obvious respiratory and gastrointestinal tract damage.There are the amount of viscous or purulent inflammatory secretions in oral or nasal in the 7 cases of disease sheep and there are hyperemia,hemorrhage,canker in the oral mucosa.Most disease sheep have different degree of diarrhea and intestinal bleeding.In addition,the each part of the liver,spleen,lungs and lymph nodes of the 7 cases of ill sheep also have important macro pathological changes.The systemic pathological changes in the diagnosis of PPR is great significance.(4)The important pathological changes of damage tissues and organs of the 7 cases of disease sheep are alterative inflammation and macrophage inflammatory.There are more quantity containing eosinophilic inclusions multinucleated giant cell in the diseased tissue,such as in the liver,spleen,lung,digestive tract and respiratory tract,etc.(5)Ultrastructural pathology research shows that the diseased organ parenchyma cell swelling,degeneration,necrosis,organelles mitochondria swelling,damage the most obvious,crest fracture,collapse or vacuoles,secondly endoplasmic reticulum expansion,rupture and collapse.The nucleus chromatin pyknosis or dissolved,late,parenchyma cell membrane rupture disintegration.Intercellular substance has a large number of mononuclear macrophage infiltration,late collagen fiber hyperplasia,partial liver tissue fibrosis.The results prove that the 7 cases of ill sheep from Ningxia Wuzhong were the ill sheep of peste des petits ruminants(PPR).On the other hand,the system analyses the characteristics of the pathological changes of the sheep of peste des petits ruminants,in order to further the diagnosis and prevention and control of the sheep of peste des petits ruminants laid a foundation.