Ectomycorrhizal Resource Survey Of Chimonobambusa Forest And The Preparation For Microbial Inoculum

Ectomycorrhizal was a kind of obligate living and nutritive fungi which formed a symbiotic body with the roots of plants.It was beneficial to promote plant growth,increase the absorption of nutrients,and stabilize the ecosystem.In this study,The macrofungi were collected from the natural woodland of Chimonobambusa hejiangensis C.D.Chu et C.S.Chao and Chimonobambusa utilis,the major species of ectomycorrhizal were identified using morphological and molecular biological methods.And the ectomycorrhizal fungi were isolated,purified and cultured.The solid fermentation method was used to prepare microbial inoculum.The main results as follows:1.A total of 34 species of macrofungi were collected from the Chimonobambusa utilis forest of Zheng an County and Chimonobambusa hejiangensis C.D.Chu et C.S.Chao forest of Ci shui county of Guizhou,10 genera,and 19 species of ectomycorrhizal were identified.There were 2 species of Entolomataceae,2 species of Lycoperdaceae,7 species of Russulaceae,1 species of Suillaceae,1 species of Cantharellaceae,1 species of Tricholomataceae and 1 species of Amanitaceae.2.Phylogenetic analysis of the ITS sequence was used to identify the ectomycorrhizal.5 of which consisitent with morphological identification,they were Entoloma murrayi,Lactarius aurantiacus,Russula lepida,Suillus bovinus and Lactarius hatsudake?3.The fruiting bodies of ectomycorrhizal were isolated and purified,and obtain2 kinds of pure mycelium.They were Russula lepida and Lactarius aurantiacus.4.The research explored the effects of different solid media on the growth of mycelia of Russula lepida.The suitable medium has been screened out:wood chips 45%,cotton hulls 45%,wheat bran 8%,1% sucrose and 1% plaster.Obtain the microbial inoculum for production.