Effects Of AM Fungi On The Growth Of Chimonobambusa Utilis

Arbuscular mycorrhizal(AM)fungi was a kind of obligate viviparous fungi and can form a symbiotic association with host plant.This kind of symbiosis can promote plant growth,improve crop yields and qualities,and improved soil structure,so the m ycorrhizal bio-fertilizers has gradually gotten popularized in the domain of agriculture and forestry.In this paper,the soil that the natural forest rhizosphere of Chimonoba mbusa utilis was collected,and the infection status of root system was determined.Th e main species and dominant species of AM fungi were identified by morphological a nd molecular biological methods.The effect that AM fungi on the growth of different growth period by inoculated with AM fungi of autochthonic(Rhizophagus intraradice s and Funneliformis mosseae)and dominant of roots(Acaulospora mellea and Scutell ospora calospora),to which gain the fine mycorrhizal.The mian results are as follows:1.Different mycorrhizal structure in all root sample was observed under microsc ope and the infection rates of Zhenan county and Tongzi county of Guizhou were hig her than 30% with used alkaline lysis and acid fuchsin staining method.The results sh owed Chimonobambusa utilis can establish a good symbiosis relation with AM fungi.2.There are 15 new species of six genera that twenty-four species of AM fungi b elonged to seven genera with based on the morphological characteristics of the isolate d spores from rhizoshere soil of Chimonobambusa utilis,which including seven speci es of Glomus,eight species of Acaulospora,one species of Sclerocystis,three species of Scutellospora,three species of Gigaspora,one species of Corymbiglomus.Phyloge netic analysis of the 18 S rDNA AML1-AML2 sequence was used to identify the AM fungi of 15 new species by nested-PCR,only two of which were consistent with morp hological identification as Glomeraceae.3.Inoculation of 4 kinds of AM fungi can promote the growth of Chimonobambusa utilis,but the effect was different because of the type of AM fungi.The result sho ws the best infection rate was Scutellospora calospora inoculated with the third leafperiod in the seeding,reached 60.49%.In the germination stage,it was best obvious that the effect of inoculation with Funneliformis mosseae on the fresh weight,which was145.71% respectively higher than CK.The net photosynthetic rate and transpirati on rate could be obviously improved by inoculation of both Funneliformis mosseae i n the germination period,which was 276.89% and 59.46% respectively higher than CK,but the chlorophyll content only increased by 53.36%.The POD activity could be obviously improved by inoculation of both Scutellospora calospora when the see dling produced the third leaf,which was 77.91% higher than CK.The SOD activity could be obviously improved by inoculation of both Rhizophagus intraradices when the seedling produced the first leaf,which was 98.21% higher than CK.Inoculation with Funneliformis mosseae in seed germination period could be significantly increased the nitrogen content of plants,which was 145.00% higher than CK.The soluble sugar content could be obviously improved by inoculation of both Scutellospora cal ospora when the seedling produced the first leaf,which was 58.50% higher than CK.In general,the effect of inoculation on the growth effect of Chimonobambusa utilis with Funneliformis mosseae and Scutellospora calospora were obvious,and it could be used as fine mycorrhizal of AM fungi which is suitable for the growth of Chimo nobambusa utilis.Test results have been clear about the location of Chimonobambusa utilis natural forest type of AM fungi in the rhizosphere and the Scutellospora calosporaominant sp ecies,screening the suitable inoculation of AM fungi strains,it will be for AM fungi i n the location of Chimonobambusa utilis plantation production application of germpla sm resources,technical support and theoretical basis.