Study On Molecular Mechanism Of Differential Development Of Nucleo-cytoplasmic Interaction In Sugarbeet

Nuclear and cytoplasmic interaction male sterility plays an important role in sugarbeet production.Revealing the mechanism of nuclear and cytoplasmic interaction male sterility provides an important theoretical basis for further research.This study was based on two dimensional electrophoresis(2-DE)combined with mass spectrometry,non-gel label free and mass spectrometry to compare the flower buds of male sterile line(N9849)and maintainer line(960766)at budding stage.The results were as follows:1.The proteomics study based on 2-DE / MS method successfully identified 21 significant differentially expressed proteins involving 8 functional categories in which the proportion of stress response functional categories was large.In the network prediction analysis,six protein spots were linked to the network,involving five functional groups: stress response,carbohydrate metabolism,regulation of growth and development,and protein synthesis.2.Study on the proteome of label-free based mass spectrometry based method to successfully identified 96 different proteins,involving 16 functional categories,including stress response,carbohydrate metabolism,pollen development etc.functional categories are in large proportion.Significant enrichment in pathway analysis to 4 pathways,Protein network prediction and analysis,Co-enriched to 14 protein spots,Seven functional groups are involved in protein synthesis and stress response.3.Among the identified differential proteins,stress response,carbohydrate metabolism,pollen development and other functional categories accounted for a large proportion.Glutathione-Stransferase,catalase and other stress-responsive proteins were significantly underexpressed in male sterile lines.Weak scavenging capacity of toxic substances and reactive oxygen species,May lead to disorder in the system of sterile lines,resulting in abortion.Malate dehydrogenase of carbohydrate metabolic functional proteins,Low expression of sucrose synthase in male sterile lines,Low carbohydrate metabolism intensity and lack of energy metabolism are also a cause of pollen infertility.Pollen development-related protein III type polyketone synthase was significantly overexpressed in the maintainer line,which maintained the fertility of the maintainer line.This study provides a theoretical basis for elucidating the mechanism and utilization of male sterility in beet at the proteomics level.