Cloning And Preliminary Functional Analysis Of SpsLAZY1a,b In Salix Psammophila

The branch angle,as an important component of plant architecture,influences the biomass,landscape and function of windbreak and sand fixation.It is regulated by endogenous genes,hormones and external enviroments,in which the known LAZY gene is involved in the plant branch angle regulation and changes the plant architecture.SpsLAZY1 a,SpsLAZY1b were cloned from S.psammophila and their sequences,protein structures were predicted by biology software.We analysed the tissue-specific expression of SpsLAZY1 a,SpsLAZY1b in different spatial patterning of branches and constructed the overexpression vectors which were transformed into Arabidopsis and 84 K poplar.The main results are as follows:1.According to the phenotypic observation and branching angle measurements,S.psammophila was divided spread type and upright type.2.SpsLAZY1 a,SpsLAZY1b were cloned from S.psammophila by homologous cloning.Both of SpsLAZY1 a and SpsLAZY1 b include 5 exons and 4 introns,their CDS similarity is 88.83%,and C-terminal amino acid residues is highly conserved.3.SpsLAZY1 a and SpsLAZY1 b were mainly expressed in the stems and leave axils from different lines of S.psammophila,and almost no expression in root.These target genes expressed highly in upright type than in spread type.4.Both of the prediction of subcellular localization and NeYFP::SpsLAZY1a,NeYFP::SpsLAZY1b transient expression to Nicotiana Benthamiana showed these genes located in the nucleus.5.The 10 lines from SpsLAZY1 a overexpression in poplar 84 K were got and 8 lines from SpsLAZY1 b overexpression in Arabidopsis.This study showed that LAZY1 gene duplication occurred in the process of evolution of S.psammophila and preliminarily reveal their phylogenetic relationship and possible function.The differential gene expression between SpsLAZY1 a and SpsLAZY1 b may be related to spacial architecture of S.psammophila.Nuclear localization indicates that their molecular function is probably related to transcriptional regulation.This study enriched the research of IGT gene family and provided a theoretical basis for application of molecular breeding of S.psammophila.