| In plants,α-1,3-fucosyltransferase catalyzes the attachment of α-1,3 fucose to N-glycans by N-glycosylation modification.Glycosylation-modified fucose is involved in hormonal regulation,secondary metabolism,detoxification,signal transduction and other functions.FUCT is an important enzyme involved in the fucosylation process,it affects the growth and development of plants by affecting the glycosylation of fucosylase.This causes tilling angle changes and cellulose synthesis.In this study,Salix psammophila was used as the research material,and design primers based on the CDS sequence of the Salix purpurea’s FUCT gene.The full-length CDS sequence of the Salix psammophila’s FUCT gene was successfully cloned by PCR and named Sps FUCT.The results of the study are as follows:1.The 1569 bp coding sequence of Salix psammophila FUCT was obtained by homologous cloning method,it encodes 522 amino acids with a molecular weight of 58.74 KD and a theoretical isoelectric point of 5.73.2.The predicted secondary structure of SpsFUCT protein is a transmembrane hydrophobic protein.It contains four glycosylation sites and no signal peptide was detected.The results of homologous protein domain and phylogenetic tree indicated that the relationship between Sps FUCT and woody plants were the closest,and the protein domain was the most similar.This protein belongs to the Glyco-transf-10 family and has a GT10 family of functional domains.3.The tissue-specific expression results showed that SpsFUCT was specifically expressed in plants and expressed the highest amount in leaves,which was twice that of leaf aphids.Its xylem expression was twice that of young stems.4.The Sp SFUCT gene’s overexpression vector was successfully constructed.The results showed that the SpsFUCT gene was successfully cloned.The function of this gene may be involved in physiological and biochemical processes such as phytohormone regulation and secondary metabolism,and has an important relationship with plant branching regulation.The results of this study enrich the study of molecular genetics of fucosyltransferases.It provides a certain foundation for studying the branching angle,branching direction,and breeding of Salix psammophila. |
