Analysis Of Pny Mutant Arabidopsis And Cloning, Tissue-Specific Expression Of PNY Gene From Salix Psammophila

As drought resistance and sand fixation trees in the northwest, Salix psammophila own unique mechanism which enables it to survive in arid desert environment. Researching on genetic mechanism of branching and wood properties development has great significance to promote the molecular breeding and culture strain with more branches, which would increase soil cover, prevent soil erosion and utilization of biology energy of Salix psammophila.In this study, mutant Arabidopsis and Salix psammophila was used as material. The phenotype of pny mutant Arabidopsis was analyzed and PNY gene was cloned from Salix psammophila. Bioinformatics and tissue-specific expression analysis of PNY gene were finished.The main results of the study were as follows:(1)We found that mutant Arabidopsis grow slowly, phenotype changed obviously and the number of branches and cauline leaves significantly increased. With the anatomy structure analysis of pny mutant Arabidopsis stem, we found that secondary xylem of stem basement was more developed than it of the wild type. Xylem cells were closer. Expansion outward and development of phloem part are faster.(2) Analyzing the conserved sequence regions and functional domains of PNY protein of Salix, we found that the protein contained 5 conserved sequence regions and 2 functional domains. Phylogenetic analysis revealed that there were closer phylogenetic relations between PNY of Salix psammophila, Salix purpurea and Populus trichocarpa.(3)Tissue-specific expression analysis of Salix PNY gene by quantitative real-time PCR showed that higher expression was detected in leaf axil, while the expression in stem, leaf, teminal bud and root were lower than that in leaf axil.The results of this study indicate that the PAT gene affected not only the development of branches and also the vascular system development. Cloned the PNY gene from Salix psammophila and it may be a transcription factor. SpsPNY gene and phylogenetic tree analysis indicated that PNY genetic relationship among woody plants were closer. Tissue-specific expression analysis of Salix PNY gene indicated that this gene might affect vascular and branches development in woody plants. This study laid the foundation for the further analysis of the function of PNY gene and has important guiding significance for the tree molecular breeding.