Effect Of Catalase Enzyme On Growth Performance,Intestinal Morphology,and Antioxidant Property Of Weaned Piglets

Abstract:This experiment was conducted to evaluate the effects of catalase enzyme?CAT?on growth performance,intestinal morphology,and antioxidant capcity of weaned piglets.240 three-line crossbred?Duroc×Landrace×Yorkshire?weaned piglets were randomly divided into 5 groups,with each group represented by 4 replicates of 12 piglets per replicate.Test group A was fed a basal diet as control group,test group B was fed the basal diet supplemented with 2 500 mg/kg ZnO,while the next 3 experimental groups?C?D?E?were fed basal diet supplemented with 1000?1500?2000 mg/kg CAT,respectively.The trial lasted for14 days.The results showed as follows:1.Compared with the control group,the feed/gain?F/G?of groups D and E were significantly respectively decreased by 3.2%?7.2%?P<0.05?,but the other growth performaces were not significantly different among all groups.2.Compared with the control group,the villus height in ileum of groups D were significantly increased?P<0.05?,and the crypt depth in ileum in group B and D were significantly decreased than those in other groups?P<0.05?.There is no significantly difference in villus height?crypt depth and villus height to crypt depth ratio?V/C?in duodenum?jejunum and ileum among other groups.3.Compared with the control group,malondialdehyde?MDA?content in serum of groups D and E were significantly decreased by 43.7%and 44.7%?P<0.05?,and glutathione?GSH?content in serum of groups B?C?D and E were significantly increased?P<0.05?;but the MDA?GSH and Superoxide dismutase?SOD?content in liver were not significantly different among all groups?P>0.05?.4.Compared with the control group,there is no significantly difference in inhibition ability of superoxide anion and hydroxyl free radical in serum among all groups?P>0.05?,except the inhibition ability of superoxide anion of groups E were significantly increased?P<0.05?.5.There is no significantly difference in the amount of Lactobacillus?Salmonella?Escherichia and total bacteria in feces of ileum ang cecum among all groups?P>0.05?.6.Compared with the control group,the apparent digestibility of ether extract of C?D?E group was significantly increased by 32.62%?33.49%?29.69%?P<0.05?.7.Compared with the control group,after 1h incubation,0.5?1?2?4 mmol/L H₂O₂significantly decrease the cell viability of porcine intestinal epithelium by 34.97%?40.2%?49.36%and 66.81%?P<0.05?,respectively;Compared with the 1 mmol/L H₂O₂ treatment,the cell viability was increased by 30.87%?39.18%and 40.85%?P<0.05?after pretreatment with 100U/mL?400U/m L?1000U/m L CAT for 4h respectively.In summary,this results indicate that the dietary supplemented with 75 U/kg CAT can decrease feed/gain?F/G?and diarrhea rate,while improve intestinal mucosal morphology,antioxidant property and Nutrient Apparent Digestibility.To some degree,dietary supplemented with CAT is comparable to the effect of the dietary supplemented with zinc oxide.