Pharmacological Study And Clinical Application Of Traditional Chinese Medicine Compound On Heat Stress

This experiment mainly carried out the traditional Chinese medicine Wang Laoji,effects of heat stress Elsholtzia powder was studied,and the prevention of broiler heat stress in clinical effects were studied.In the first part experiment,80 male Wistar rats were randomly divided into four groups,20 rats in each group,divided into groups,Elsholtzia powder group,because of heat stress in positive control group(referred to as heat stress group)and temperature group,respectively to two Chinese test group rats at 0.006 mL/g BW perfusion of compound oral liquid the 7 day trial period.3h,1d,3d and 7d were used to detect the rectal temperature,the number of white blood cells in peripheral blood,blood biochemistry,serum HSP70 and cortisol levels,antioxidant function and the histological structure of small intestine in rats.The test results show that the Wang Laoji and Elsholtzia powder can relieve rat heat stress at elevated temperature,the heat stress rat blood biochemical indexes tended to normal physiological level.Elevated Wang Laoji and Elsholtzia powder oral liquid could inhibit rats under heat stress of serum HSP70 and Cor levels,and increase the antioxidative function,inhibit the formation of MDA.Heat stress rat intestinal villus degeneration,cracking,shedding of epithelial cells degeneration,hyperplasia of inflammatory cells in the local area,group and group of Elsholtzia splendens.Heat stress rat small intestinal structure has certain protective effect.The second part of the test,180 14 day old chickens were randomly divided into four groups,each group of 45 birds,respectively Wang Laoji group,Elsholtzia powder group,positive control group(referred to as heat stress and heat stress group)and temperature group,0.5% Chinese medicine powder were added two Chinese test group of chicken in drinking water.1d,3d,7d,14 d,28d were randomly sampled from 6 chickens in each group,and the growth performance,blood biochemistry,serum HSP70 and cortisol levels and antioxidant function were measured.Heat stress in each stage of broiler average daily gain was significantly reduced,the reduction of food intake,feed weight ratio increased significantly,and the addition of Elsholtzia drinking water Wanglaoji oral liquid can increase the bulk weight gain,feed intake,feed weight ratio was reduced,but the effect is less than optimum temperature group.At 28 d heat stress,heat stress group on the activity of AST was significantly(P<0.01),ALT activity increased,Elsholtzia powder group broiler GLU was significantly higher than that of heat stress group(P<0.05);heat stress group chicken heat shock protein increased,serum HSP70 content was significantly lower than that of Wang Ji Xiang Ru powder group(P<0.05),and Elsholtzia powder of serum levels of HSP70 and temperature group increased significantly(P<0.01).Heat stress group serum Cor content was higher than the optimum temperature control group,adding C hinese herbal compound Wang Laoji and Elsholtzia powder can inhibit the serum Cor in the drinking water,and Wang Laoji is better than that of Elsholtzia splendens.Wang Laoji group T-AOC was significantly higher than that of heat stress group(P<0.01),temperature group and Wang Laoji group SOD were significantly higher than those of heat stress group(P<0.05),the activity of SOD was significantly higher than that of Elsholtzia splende ns heat stress group(P<0.05),and the heat stress 14 d,Elsholtzia powder group serum GSH-Px activity was significantly higher than that of heat stress group and temperature group(P<0.05),heat stress group MDA content than the other three groups with high MDA content.At 28 d heat stress;heat stress group cecal villi shorter deletion,fracture,disorder,inflammatory cell hyperplasia group and severe;Wong Lo Kat Elsholtzia powder group broiler cecum intestinal villus arrangement is neat,no obvious tissue pathological changes.