Study On The Preventive Effect Of Taurine On Fatty Liver Hemorrhagic Syndrome In Laying Hens

AIM: Fatty Liver Hemorrhage Syndrome(FLHS)resulting in a sharp decline in egg production in laying hens,reduced productivity and increased mortality in groups,causes serious economic losses of poultry husbandry.In the present study,FLHS model of laying hens was established,and taurine was administered to investigate the preventive effect of taurine on FLHS in laying hens,andtries to interprete the mechanism from the point of mitochondria protection,in order to provide scientific basis for the application of taurine to the prevention of FLHS in laying hens and mitochondrion-related diseases.METHODS: 300 Hyland brown laying hens with the age of 300 days were selected.They are randomly divided into 6 groups: normal control group(C),taurine control group I(T1),taurine control group II(T2),FLHS model group(M),taurine prevention group I(MT1),and taurine prevention group II(MT2).The C,T1,and T2 groups are fed with basal diet,and the M,MT1,and MT2 groups were fed with high-energy and low-protein diets,among wichi,T1,T2,MT1 and MT2 groups were administered with 0.05% or 3% taurine in diets.The experiment was lasted for 16 weeks.Then the laying hens are sacrificed,serum and liver were collected.Paraffin sections were prepeared and pathological changes were observed after HE staining.Serum activities of alanine aminotransferase(ALT)and aspartate aminotransferase(AST),serum and hepatic concentrations of total triglyceride(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),malondialdehyde(MDA);the activity of total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-Px)were measured.The liver mitochondria was isolated,and mitochondrial membrane potential was detected by JC-1 method;the activities of catalase(CAT),mitochondrial marker enzyme citrate synthase(CS,carnitine palmitoyltransferase(CPT-1)and cytochrome c oxidase(COX)are determined by ABC-ELISA.Total RNA were extracted from the liver,the mRNA expression of mitochondrial functional enzymes and mitochondrial dynamics related factors were detected by qRT-PCR.RESULT: In this experiment,the FLHS model of laying hens is successfully established by feeding high energy and low protein diets,the incidence of FLHS in the model group increased significantly.In the FLHS model group,obvious hepatic steatosis is observed,and the activities of ALT and AST,the concentrations of TC,TG,LDL-C and MDAwere extremely significant increased(p<0.01),while HDL-C was extremely significant decreased(p<0.01);The content and the activities of T-SOD and GSH-Px in the model group were significantly increased(p<0.01);Meanwhile,the liver mitochondrial membrane potential,the activities of CS,COX,CAT and CPT-1 were extreme significantly reduced(p<0.01);Mfn1,Mfn2,OPA1,CAT,MnSOD,CPT-1 mRNA expression levels were also decreased significantly(p<0.01).Compared with the FLHS model group,the ALT and AST activities and the contents of TC,TG,LDL-C,the content of MDA in the taurine preventive groups were extremely significant increased(p<0.01),while the contents of HDL-C,the activities of T-SOD and GSH-Px wereextremely significant increased(p<0.01).The mitochondrial membrane potential,the activity of CS,CAT,COX and CPT-1 were all increased extremely significant(p<0.01);,and the mRNA expression levels of MnSOD,CAT,CPT-1,Mfn1,Mfn2,and OPA1 are obviously up-regulated(p<0.01).CONCLUSION: 1.In this study,FLHS model was successfully established by using high energy and low protein diets.2.The results of this study indicate that taurine can protect liver cells,reduce lipid deposition,increase the antioxidant capacity of liver,improve mitochondrial dysfunction,maintain mitochondrial dynamic balance,and thus have a preventive effect on FLHS in laying hens.